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题名: Spatial Profiling of Gibberellins in a Single Leaf Based on Microscale Matrix Solid-Phase Dispersion and Precolumn Derivatization Coupled with Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry
作者: Deng, Ting1, 2;  Wu, Dapeng1;  Duan, Chunfeng1;  Yan, Xiaohui1;  Du, Yan1, 2;  Zou, Jun1, 2;  Guan, Yafeng1
刊名: ANALYTICAL CHEMISTRY
发表日期: 2017-09-05
DOI: 10.1021/acs.analchem.7b02589
卷: 89, 期:17, 页:9537-9543
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A spatial-resolved analysis method for profiling of gibberellins (GAs) in a single leaf was developed on the basis of microscale sample preparation and precolumn derivatization coupled with ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The proposed microscale sample preparation was based on modified matrix solid-phase dispersion (MSPD) method, in which the plant sample (<1 mg) and C18 sorbent were ground together in one microcentrifuge tube, and then extraction solvent was added followed by centrifugation. In this protocol, the grinding, extraction, and purification were performed in one microcentrifuge tube-without any sample transfer step, resulting in an obvious decrease in sample loss. Moreover, a "new derivatization reagent, 3-bromopropyltrimethylaimnonium bromide (BPTAB), was used to further enhance the signal intensities of GAs on MS by 3-4 orders of magnitude, which was much higher than the reported derivatization reagents for GAs such as bromocholine bromide and 3-bromoactonyltrimethylammonium bromide. The present method showed high sensitivity (minimum detectable amount (MDA) of 10.1-72.3 amol for eight GAs) and low sample consumption (down to 0.30 mg FW). Under the optimized conditions, the distribution of GA(19) in a single Arabidopsis thaliana leaf was profiled with a spatial resolution of 2 X 2 mm(2).
关键词[WOS]: PLANT HORMONES ;  ACIDIC PHYTOHORMONES ;  ENDOGENOUS BRASSINOSTEROIDS ;  QUANTITATIVE-ANALYSIS ;  ARABIDOPSIS-THALIANA ;  FRESH PLANT ;  EXTRACTION ;  QUANTIFICATION ;  GROWTH ;  RICE
语种: 英语
WOS记录号: WOS:000410014900130
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/149902
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China

Recommended Citation:
Deng, Ting,Wu, Dapeng,Duan, Chunfeng,et al. Spatial Profiling of Gibberellins in a Single Leaf Based on Microscale Matrix Solid-Phase Dispersion and Precolumn Derivatization Coupled with Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2017,89(17):9537-9543.
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