DICP OpenIR
Thiol-yne click synthesis of boronic acid functionalized silica nanoparticle-graphene oxide composites for highly selective enrichment of glycoproteins
Yang, Jingai1; He, Xiwen1; Chen, Langxing1,2; Zhang, Yukui1,3
关键词Graphene Oxide Thiol-yne Click Reaction Boronate Affinity Glycoprotein
刊名JOURNAL OF CHROMATOGRAPHY A
2017-09-01
DOI10.1016/j.chroma.2017.07.026
1513页:118-125
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]TRANSFER RADICAL POLYMERIZATION ; MOLECULARLY IMPRINTED POLYMERS ; SOLID-PHASE EXTRACTION ; MAGNETIC NANOPARTICLES ; MONOLITHIC COLUMN ; GRAPHITE OXIDE ; LIQUID-CHROMATOGRAPHY ; GLYCOPEPTIDES ; CHEMISTRY ; GLYCOSYLATION
英文摘要In this work, a facile novel strategy is developed for the preparation of 4-mercapto-phenylboronic acid functionalized silica nanoparticle-graphene oxide composite (GO@MPBA) via thiol-yne click reaction. The morphology, structure and composition of the resulting GO@MPBA was characterized by transmission electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, Fourier transform Raman spectroscopy, Thermogravimetric analysis and X-ray photoelectron spectrometry. GO@MPBA exhibited the high binding capacity towards glycoproteins such as ovalbumin (1288.8 mg g(-1)), immunoglobulin G (1144.1 mg g(-1)), transferrin (592.1 mg g(-1)) and horseradish peroxidase (392.4 mg g(-1)), in contrast, the binding capacity of each non-glycoproteins (cytochrome c, deoxyribonuclease A, pepsin, trypsin, lysozyme and bovine serum albumin) is less than 50 mg g-1. Furthermore, the GO@MPBA still remained a good binding capacity after the five times of adsorption-desorption cycles. The selective enrichment of glycoproteins from real egg white samples by GO@MPBA was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. This work could present a facile novel approach of surface modification to design environmentally friendly and more efficient adsorbents for the isolation and enrichment of glycoprotein from complex biological samples. (C) 2017 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000408288400013
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/149903
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin Key Lab Biosensing & Mol Recognit, Res Ctr Analyt Sci,Coll Chem, Tianjin 300071, Peoples R China
2.Nankai Univ, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Yang, Jingai,He, Xiwen,Chen, Langxing,et al. Thiol-yne click synthesis of boronic acid functionalized silica nanoparticle-graphene oxide composites for highly selective enrichment of glycoproteins[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1513:118-125.
APA Yang, Jingai,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2017).Thiol-yne click synthesis of boronic acid functionalized silica nanoparticle-graphene oxide composites for highly selective enrichment of glycoproteins.JOURNAL OF CHROMATOGRAPHY A,1513,118-125.
MLA Yang, Jingai,et al."Thiol-yne click synthesis of boronic acid functionalized silica nanoparticle-graphene oxide composites for highly selective enrichment of glycoproteins".JOURNAL OF CHROMATOGRAPHY A 1513(2017):118-125.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, Jingai]的文章
[He, Xiwen]的文章
[Chen, Langxing]的文章
百度学术
百度学术中相似的文章
[Yang, Jingai]的文章
[He, Xiwen]的文章
[Chen, Langxing]的文章
必应学术
必应学术中相似的文章
[Yang, Jingai]的文章
[He, Xiwen]的文章
[Chen, Langxing]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。