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题名: Maleic Anhydride Labeling-Based Approach for Quantitative Proteomics and Successive Derivatization of Peptides
作者: Tian, Shanshan1;  Zheng, Shuzhen2;  Han, Yanpu2;  Guo, Zhenchang1;  Zhai, Guijin1;  Bai, Xue1;  He, Xiwen2;  Fan, Enguo3, 4;  Zhang, Yukui2, 5;  Zhang, Kai1
刊名: ANALYTICAL CHEMISTRY
发表日期: 2017-08-15
DOI: 10.1021/acs.analchem.7b01120
卷: 89, 期:16, 页:8259-8265
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Chemical derivatization is a simple approach for stable-isotope covalent labeling of proteins in quantitative proteomics. Herein we describe the development of a novel maleyl-labeling-based approach for protein quantification. Under optimized conditions, maleic anhydride can serve as a highly efficient reagent to label the amino groups of tryptic peptides. Furthermore, "click chemistry" was successfully applied to obtain the second modification of maleylated peptides via thiol-Michael addition reaction. Accurate quantification was further achieved via the first or/and second step stable-isotope labeling in this study. Our data thus demonstrate that the maleyl-labeling-based method is simple, accurate, and reliable for quantitative proteomics. The developed method not only enables an enhanced sequence coverage of proteins by improving the identification of small and hydrophilic peptides, but also enables a controllable, successive, second derivatization of labeled peptides or proteins, and therefore holds a very promising potential for in-depth analysis of protein structures and dynamics.
关键词[WOS]: MASS-SPECTROMETRY ;  QUANTIFICATION ;  CHEMISTRY ;  SILAC ;  TAGS ;  TOOL
语种: 英语
WOS记录号: WOS:000407988600013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/149915
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Tianjin Med Univ, Collaborat Innovat Ctr Tianjin Med Epigenet 2011, Minist Educ,Dept Biochem & Mol Biol, Tianjin Key Lab Med Epigenet,Key Lab Immune Micro, Tianjin 300070, Peoples R China
2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
3.Univ Freiburg, Inst Biochem & Mol Biol, Stefan Meier Str 17, D-79104 Freiburg, Germany
4.Chinese Acad Med Sci, Peking Union Med Coll, Sch Basic Med, Dept Microbiol & Parasitol,Inst Basic Med Sci, Beijing 100005, Peoples R China
5.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Liaoning, Peoples R China

Recommended Citation:
Tian, Shanshan,Zheng, Shuzhen,Han, Yanpu,et al. Maleic Anhydride Labeling-Based Approach for Quantitative Proteomics and Successive Derivatization of Peptides[J]. ANALYTICAL CHEMISTRY,2017,89(16):8259-8265.
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