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题名: Hydrogen bond based smart polymer for highly selective and tunable capture of multiply phosphorylated peptides
作者: Qing, Guangyan1;  Lu, Qi1;  Li, Xiuling2;  Liu, Jing2;  Ye, Mingliang2;  Liang, Xinmiao2;  Sun, Taolei1, 3
刊名: NATURE COMMUNICATIONS
发表日期: 2017-09-06
DOI: 10.1038/s41467-017-00464-0
卷: 8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Multisite phosphorylation is an important and common mechanism for finely regulating protein functions and subsequent cellular responses. However, this study is largely restricted by the difficulty to capture low-abundance multiply phosphorylated peptides (MPPs) from complex biosamples owing to the limitation of enrichment materials and their interactions with phosphates. Here we show that smart polymer can serve as an ideal platform to resolve this challenge. Driven by specific but tunable hydrogen bonding interactions, the smart polymer displays differential complexation with MPPs, singly phosphorylated and non-modified peptides. Importantly, MPP binding can be modulated conveniently and precisely by solution conditions, resulting in highly controllable MPP adsorption on material surface. This facilitates excellent performance in MPP enrichment and separation from model proteins and real biosamples. High enrichment selectivity and coverage, extraordinary adsorption capacities and recovery towards MPPs, as well as high discovery rates of unique phosphorylation sites, suggest its great potential in phosphoproteomics studies.
关键词[WOS]: MULTISITE PHOSPHORYLATION ;  AFFINITY-CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  PHOSPHOPEPTIDE ENRICHMENT ;  PROTEIN-PHOSPHORYLATION ;  PHOSPHOPROTEOMICS ;  IDENTIFICATION ;  PROTEOMICS ;  TYROSINE ;  DISEASE
语种: 英语
WOS记录号: WOS:000409458000015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/149966
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, 122 Luoshi Rd, Wuhan 430070, Hubei, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China
3.Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, 122 Luoshi Rd, Wuhan 430070, Hubei, Peoples R China

Recommended Citation:
Qing, Guangyan,Lu, Qi,Li, Xiuling,et al. Hydrogen bond based smart polymer for highly selective and tunable capture of multiply phosphorylated peptides[J]. NATURE COMMUNICATIONS,2017,8.
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