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题名: Chloroplasts Isolation from Chlamydomonas reinhardtii under Nitrogen Stress
作者: Yang, Miao1, 2, 3;  Jiang, Jun-Peng1, 2;  Xie, Xi4;  Chu, Ya-Dong1;  Fan, Yan1, 2;  Cao, Xu-Peng1;  Xue, Song1;  Chi, Zhan-You3
关键词: Chlamydomonas ;  chloroplast isolation ;  triacylglycerol ;  nitrogen stress ;  fatty acid biomarkers
刊名: FRONTIERS IN PLANT SCIENCE
发表日期: 2017-08-29
DOI: 10.3389/fpls.2017.01503
卷: 8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Plant Sciences
研究领域[WOS]: Plant Sciences
英文摘要: Triacylglycerols are produced in abundance through chloroplast and endoplasmic reticulum pathways in some microalgae exposed to stress, though the relative contribution of either pathway remains elusive. Characterization of these pathways requires isolation of the organelles. In this study, an efficient and reproducible approach, including homogenous batch cultures of nitrogen-deprived algal cells in photobioreactors, gentle cell disruption using a simple custom-made disruptor with mechanical shear force, optimized differential centrifugation and Percoll density gradient centrifugation, was developed to isolate chloroplasts from Chlamydomonas reinhardtii subjected to nitrogen stress. Using this approach, the maximum limited stress duration was 4 h and the stressed cells exhibited 19 and 32% decreases in intracellular chlorophyll and nitrogen content, respectively. Chloroplasts with 48 - 300 mu g chlorophyll were successfully isolated from stressed cells containing 10 mg chlorophyll. These stressed chloroplasts appeared intact, as monitored by ultrastructure observation and a novel quality control method involving the fatty acid biomarkers. This approach can provide sufficient quantities of intact stressed chloroplasts for subcellular biochemical studies in microalgae.
关键词[WOS]: INTACT CHLOROPLASTS ;  INORGANIC CARBON ;  HEAT-STRESS ;  LOW CO2 ;  CELLS ;  BIOSYNTHESIS ;  LIPIDS ;  PHOTOSYNTHESIS ;  FRACTIONATION ;  ACCUMULATION
语种: 英语
WOS记录号: WOS:000408660800001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/149974
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian, Peoples R China
4.Liaoning Ocean & Fisheries Sci Res Inst, Dalian, Peoples R China

Recommended Citation:
Yang, Miao,Jiang, Jun-Peng,Xie, Xi,et al. Chloroplasts Isolation from Chlamydomonas reinhardtii under Nitrogen Stress[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
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