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题名: Bioinspired onion epithelium-like structure promotes the maturation of cardiomyocytes derived from human pluripotent stem cells
作者: Xu, Cong1, 2;  Wang, Li1, 2;  Yu, Yue1, 2;  Yin, Fangchao1, 2;  Zhang, Xiaoqing1;  Jiang, Lei1, 2;  Qin, Jianhua1, 2
刊名: BIOMATERIALS SCIENCE
发表日期: 2017-09-01
DOI: 10.1039/c7bm00132k
卷: 5, 期:9, 页:1810-1819
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Materials Science, Biomaterials
研究领域[WOS]: Materials Science
英文摘要: Organized cardiomyocyte alignment is critical to maintain the mechanical properties of the heart. In this study, we present a new and simple strategy to fabricate a biomimetic microchip designed with an onion epithelium-like structure and investigate the guided behavior of human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) on the substrate. The hiPSC-CMs were observed to be confined by the three dimensional surficial features morphologically, analogous to the in vivo microenvironment, and exhibited an organized anisotropic alignment on the onion epithelium-like structure with good beating function. The calcium imaging of hiPSC-CMs demonstrated a more mature Ca2+ spark pattern as well. Furthermore, the expression of sarcomere genes (TNNI3, MYH6 and MYH7), potassium channel genes (KCNE1 and KCNH2), and calcium channel genes (RYR2) was significantly up-regulated on the substrate with an onion epithelium-like structure instead of the surface without the structure, indicating a more matured status of cardiomyocytes induced by this structure. It appears that the biomimetic micro-patterned structure, analogous to in vivo cellular organization, is an important factor that might promote the maturation of hiPSC-CMs, providing new biological insights to guide hiPSC-CM maturation by biophysical factors. The established approach may offer an effective in vitro model for investigating cardiomyocyte differentiation, maturation and tissue engineering applications.
关键词[WOS]: FUNCTIONAL MATURATION ;  HUMAN MYOCARDIUM ;  CULTURE MODEL ;  BETA-CATENIN ;  HEART ;  GENERATION ;  CONTRACTILITY ;  GROWTH ;  CHIP ;  CUES
语种: 英语
WOS记录号: WOS:000408253000011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/149978
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China

Recommended Citation:
Xu, Cong,Wang, Li,Yu, Yue,et al. Bioinspired onion epithelium-like structure promotes the maturation of cardiomyocytes derived from human pluripotent stem cells[J]. BIOMATERIALS SCIENCE,2017,5(9):1810-1819.
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