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题名: Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation
作者: Lin, Xinping1;  Gao, Ning1;  Liu, Sasa1;  Zhang, Sufang2;  Song, Shuang1;  Ji, Chaofan1;  Dong, Xiuping1;  Su, Yichen3;  Zhao, Zongbao Kent2;  Zhu, Beiwei1
关键词: Rhodosporidium toruloides ;  carotenoids ;  Agrobacterium tumefaciens-mediated transformation ;  T-DNA insertion mutants
刊名: YEAST
发表日期: 2017-08-01
DOI: 10.1002/yea.3236
卷: 34, 期:8, 页:335-342
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Microbiology ;  Mycology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Microbiology ;  Mycology
英文摘要: The red yeast Rhodosporidium toruloides is a known lipid producer capable of accumulating large amounts of triacylglycerols and carotenoids. However, it remains challenging to study its carotenoid production profiles owing to limited biochemical information and inefficient genetic tools. Here we used an Agrobacterium tumefaciensmediated transformation (ATMT) to change its carotenoid production and profiles. We constructed R. toruloides NP11 mutant libraries with ATMT, selected three mutants with different colours, characterized their carotenoid products by high-pressure liquid chromatography-mass spectrometry (HPLC-MS) analysis and assured differences among those strains in terms of carotenoid production and its composition profiles. We then located T-DNA insertion sites using the genome walking technology and provided discussions in terms of the new phenotypes. This study is the first of its kind to change the carotenoid production profiles in R. toruloides. Copyright (C) 2017 John Wiley & Sons, Ltd.
关键词[WOS]: BY-PRODUCTS ;  RHODOTORULA ;  YEASTS ;  EFFICIENCY ;  GLYCEROL ;  LIPIDS ;  FOOD
语种: 英语
WOS记录号: WOS:000409009500002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/149981
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Sch Food Sci & Technol, Dalian 116034, Peoples R China
2.Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
3.Oregon State Univ, Seafood Res & Educ Ctr, Astoria, OR 97103 USA

Recommended Citation:
Lin, Xinping,Gao, Ning,Liu, Sasa,et al. Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation[J]. YEAST,2017,34(8):335-342.
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