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Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation
Lin, Xinping1; Gao, Ning1; Liu, Sasa1; Zhang, Sufang2; Song, Shuang1; Ji, Chaofan1; Dong, Xiuping1; Su, Yichen3; Zhao, Zongbao Kent2; Zhu, Beiwei1
关键词Rhodosporidium Toruloides Carotenoids Agrobacterium Tumefaciens-mediated Transformation T-dna Insertion Mutants
刊名YEAST
2017-08-01
DOI10.1002/yea.3236
34期:8页:335-342
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology
关键词[WOS]BY-PRODUCTS ; RHODOTORULA ; YEASTS ; EFFICIENCY ; GLYCEROL ; LIPIDS ; FOOD
英文摘要The red yeast Rhodosporidium toruloides is a known lipid producer capable of accumulating large amounts of triacylglycerols and carotenoids. However, it remains challenging to study its carotenoid production profiles owing to limited biochemical information and inefficient genetic tools. Here we used an Agrobacterium tumefaciensmediated transformation (ATMT) to change its carotenoid production and profiles. We constructed R. toruloides NP11 mutant libraries with ATMT, selected three mutants with different colours, characterized their carotenoid products by high-pressure liquid chromatography-mass spectrometry (HPLC-MS) analysis and assured differences among those strains in terms of carotenoid production and its composition profiles. We then located T-DNA insertion sites using the genome walking technology and provided discussions in terms of the new phenotypes. This study is the first of its kind to change the carotenoid production profiles in R. toruloides. Copyright (C) 2017 John Wiley & Sons, Ltd.
语种英语
WOS记录号WOS:000409009500002
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/149981
专题中国科学院大连化学物理研究所
作者单位1.Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Sch Food Sci & Technol, Dalian 116034, Peoples R China
2.Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
3.Oregon State Univ, Seafood Res & Educ Ctr, Astoria, OR 97103 USA
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Lin, Xinping,Gao, Ning,Liu, Sasa,et al. Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation[J]. YEAST,2017,34(8):335-342.
APA Lin, Xinping.,Gao, Ning.,Liu, Sasa.,Zhang, Sufang.,Song, Shuang.,...&Zhu, Beiwei.(2017).Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation.YEAST,34(8),335-342.
MLA Lin, Xinping,et al."Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation".YEAST 34.8(2017):335-342.
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