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题名: Synthesis of polymeric monoliths via thiol-maleimide polymerization reaction for highly efficient chromatographic separation
作者: Bai, Jingyao1, 2;  Ou, Junjie1;  Zhang, Haiyang1, 2;  Ma, Shujuan1, 2;  Shen, Yehua2;  Ye, Mingliang1
关键词: Polymeric monolith ;  Thiol-maleimide ;  Capillary liquid chromatography ;  cLC-MS
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2017-09-08
DOI: 10.1016/j.chroma.2017.07.070
卷: 1514, 页:72-79
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: One-step thiol-maleimide polymerization reaction was firstly adopted for direct preparation of polymeric monoliths via alkaline-catalyzed reaction of 4,4'-bis(maleimidophenyl)methane (BMI) and trimethylolpropane tris(3-mercaptopropionate) (3SH)/pentaerythriol tetra(3-mercaptopropionate) (4SH) in the presence of a small amount of triethylamine (TEA). The polymerization could be performed within 3 h, which was faster than thermal-initiated free radical polymerization. Two kinds of monoliths, poly(BMI-co-3SH) (marked as I) and poly(BMI-co-4SH) (marked as II), were characterized with scanning electron microscopy (SEM), attenuated total reflection Fourier-transformed infrared spectroscopy (ATR-FTIR), thermal gravimetric analysis (TGA) and mercury intrusion porosimetry (MIP). Satisfactory chromatographic separation ability and column efficiency were gained for analysis of small molecular compounds such as alkylbenzenes, polynuclear aromatic hydrocarbons (EPA 610) and phenols in reversed-phase capillary liquid chromatography (cLC). High column efficiency (180,500 N/m) for butylbenzene was acquired on poly(BMI-co-3SH) column I-2, which was higher than those on most reported polymeric monoliths. A retention-independent efficient performance of small molecules was obtained by plotting of plate height (H) of alkylbenzenes versus the linear velocity (u). A term values in van Deemter equation of I-2 (1.72-0.24 mu m) and poly(BMI-co-4SH) column II-2 (5.28-4.14 mu m) were smaller than those of traditional organic/hybrid monoliths. Finally, as a practical application, 53 and 2184 unique peptides from the tryptic digests of bovine serum albumin (BSA) and HeLa cell proteins were positively identified with poly(BMI-co-3SH) monolith in cLC-MS. (C) 2017 Published by Elsevier B.V.
关键词[WOS]: CAPILLARY LIQUID-CHROMATOGRAPHY ;  ENE CLICK CHEMISTRY ;  SKELETAL HYBRID MONOLITHS ;  SMALL MOLECULES ;  AFFINITY-CHROMATOGRAPHY ;  COLUMNS ;  PERFORMANCE ;  PEPTIDE ;  PROTEIN ;  PARTICLES
语种: 英语
WOS记录号: WOS:000409153600007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/149988
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Northwest Univ, Key Lab Synthet & Nat Funct Mol Chem, Minist Educ,Key Lab Yulin Desert Plants Resources, Coll Chem & Mat Sci,Shaanxi Alcohol Ether & Bioma, Xian 710069, Peoples R China

Recommended Citation:
Bai, Jingyao,Ou, Junjie,Zhang, Haiyang,et al. Synthesis of polymeric monoliths via thiol-maleimide polymerization reaction for highly efficient chromatographic separation[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1514:72-79.
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