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题名: A novel substrate-inspired fluorescent probe to monitor native albumin in human plasma and living cells
作者: Ge, Guang-Bo1, 2;  Feng, Lei3, 4;  Jin, Qiang2, 5;  Wang, Yi-Ru2, 4;  Liu, Zhao-Ming1, 2;  Zhu, Xin-Yue6;  Wang, Ping1, 2;  Hou, Jie3, 4;  Cui, Jing-Nan3;  Yang, Ling1, 2
关键词: Human albumin ;  Fluorescent probe ;  Pseudo-esterase activity ;  Living cells ;  Two-photon imaging
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2017-10-09
DOI: 10.1016/j.aca.2017.07.048
卷: 989, 页:71-79
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Human albumin (HA) displays crucial roles in maintaining health and fighting diseases. Accurate determination of native HA in plasma or non-plasma samples are of immense significance in both basic research and clinical practice. Herein, a novel ratiometric two-photon fluorescent probe (N-butyl-4-(4-phenyl-benzoyloxy) 1,8-naphthalimide, BPBN) has been designed and developed for highly selective and sensitive sensing the enzymatic activities of HA, on the basis of its unique pseudo-esterase feature. BPBN exhibits excellent selectivity, high sensitivity and good reactivity under physiological conditions. As an enzymatic activity-based probe, BPBN can distinguish between native HA and denatured HA, while the currently used dye-binding method cannot. The probe has been successfully applied to measure native HA in plasma samples and the secreted HA in the hepatocyte culture supernatant. BPBN has also been used for two-photon imaging of HA reabsorption in living renal cells, and the results demonstrate that this probe exhibits good cell permeability, low cytotoxicity and high imaging resolution. All these findings suggest that BPBN can be reliably used for the highly selective and sensitive detection of native HA in complex biological samples, as well as for exploring HA-associated biological processes and the physiological functions of native HA in living cells. (C) 2017 Elsevier B.V. All rights reserved.
关键词[WOS]: HUMAN-SERUM-ALBUMIN ;  COMPLEX BIOLOGICAL SAMPLES ;  ESTERASE-LIKE ACTIVITY ;  LIGAND-BINDING ;  ENZYMATIC-PROPERTIES ;  CARBOXYLESTERASE 2 ;  BROMCRESOL PURPLE ;  PRACTICAL ASPECTS ;  DESIGN ;  SENSOR
语种: 英语
WOS记录号: WOS:000410343100008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150001
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Shanghai Univ Tradit Chinese Med, Inst Interdisciplinary Med, Shanghai 201203, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
4.Dalian Med Univ, Dalian 116044, Peoples R China
5.Zhengzhou Univ, Affiliated Hosp 1, Zhengzhou, Henan, Peoples R China
6.Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou, Gansu, Peoples R China

Recommended Citation:
Ge, Guang-Bo,Feng, Lei,Jin, Qiang,et al. A novel substrate-inspired fluorescent probe to monitor native albumin in human plasma and living cells[J]. ANALYTICA CHIMICA ACTA,2017,989:71-79.
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