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Spatial Profiling of Gibberellins in a Single Leaf Based on Microscale Matrix Solid-Phase Dispersion and Precolumn Derivatization Coupled with Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry
Deng, Ting1,2; Wu, Dapeng1; Duan, Chunfeng1; Yan, Xiaohui1; Du, Yan1,2; Zou, Jun1,2; Guan, Yafeng1
刊名ANALYTICAL CHEMISTRY
2017-09-05
DOI10.1021/acs.analchem.7b02589
89期:17页:9537-9543
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PLANT HORMONES ; ACIDIC PHYTOHORMONES ; ENDOGENOUS BRASSINOSTEROIDS ; QUANTITATIVE-ANALYSIS ; ARABIDOPSIS-THALIANA ; FRESH PLANT ; EXTRACTION ; QUANTIFICATION ; GROWTH ; RICE
英文摘要A spatial-resolved analysis method for profiling of gibberellins (GAs) in a single leaf was developed on the basis of microscale sample preparation and precolumn derivatization coupled with ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The proposed microscale sample preparation was based on modified matrix solid-phase dispersion (MSPD) method, in which the plant sample (<1 mg) and C18 sorbent were ground together in one microcentrifuge tube, and then extraction solvent was added followed by centrifugation. In this protocol, the grinding, extraction, and purification were performed in one microcentrifuge tube-without any sample transfer step, resulting in an obvious decrease in sample loss. Moreover, a "new derivatization reagent, 3-bromopropyltrimethylaimnonium bromide (BPTAB), was used to further enhance the signal intensities of GAs on MS by 3-4 orders of magnitude, which was much higher than the reported derivatization reagents for GAs such as bromocholine bromide and 3-bromoactonyltrimethylammonium bromide. The present method showed high sensitivity (minimum detectable amount (MDA) of 10.1-72.3 amol for eight GAs) and low sample consumption (down to 0.30 mg FW). Under the optimized conditions, the distribution of GA(19) in a single Arabidopsis thaliana leaf was profiled with a spatial resolution of 2 X 2 mm(2).
语种英语
WOS记录号WOS:000410014900130
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/150002
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
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Deng, Ting,Wu, Dapeng,Duan, Chunfeng,et al. Spatial Profiling of Gibberellins in a Single Leaf Based on Microscale Matrix Solid-Phase Dispersion and Precolumn Derivatization Coupled with Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2017,89(17):9537-9543.
APA Deng, Ting.,Wu, Dapeng.,Duan, Chunfeng.,Yan, Xiaohui.,Du, Yan.,...&Guan, Yafeng.(2017).Spatial Profiling of Gibberellins in a Single Leaf Based on Microscale Matrix Solid-Phase Dispersion and Precolumn Derivatization Coupled with Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry.ANALYTICAL CHEMISTRY,89(17),9537-9543.
MLA Deng, Ting,et al."Spatial Profiling of Gibberellins in a Single Leaf Based on Microscale Matrix Solid-Phase Dispersion and Precolumn Derivatization Coupled with Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry".ANALYTICAL CHEMISTRY 89.17(2017):9537-9543.
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