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题名: Thiol-yne click synthesis of boronic acid functionalized silica nanoparticle-graphene oxide composites for highly selective enrichment of glycoproteins
作者: Yang, Jingai1;  He, Xiwen1;  Chen, Langxing1, 2;  Zhang, Yukui1, 3
关键词: Graphene oxide ;  Thiol-yne click reaction ;  Boronate affinity ;  Glycoprotein
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2017-09-01
DOI: 10.1016/j.chroma.2017.07.026
卷: 1513, 页:118-125
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In this work, a facile novel strategy is developed for the preparation of 4-mercapto-phenylboronic acid functionalized silica nanoparticle-graphene oxide composite (GO@MPBA) via thiol-yne click reaction. The morphology, structure and composition of the resulting GO@MPBA was characterized by transmission electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, Fourier transform Raman spectroscopy, Thermogravimetric analysis and X-ray photoelectron spectrometry. GO@MPBA exhibited the high binding capacity towards glycoproteins such as ovalbumin (1288.8 mg g(-1)), immunoglobulin G (1144.1 mg g(-1)), transferrin (592.1 mg g(-1)) and horseradish peroxidase (392.4 mg g(-1)), in contrast, the binding capacity of each non-glycoproteins (cytochrome c, deoxyribonuclease A, pepsin, trypsin, lysozyme and bovine serum albumin) is less than 50 mg g-1. Furthermore, the GO@MPBA still remained a good binding capacity after the five times of adsorption-desorption cycles. The selective enrichment of glycoproteins from real egg white samples by GO@MPBA was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. This work could present a facile novel approach of surface modification to design environmentally friendly and more efficient adsorbents for the isolation and enrichment of glycoprotein from complex biological samples. (C) 2017 Elsevier B.V. All rights reserved.
关键词[WOS]: TRANSFER RADICAL POLYMERIZATION ;  MOLECULARLY IMPRINTED POLYMERS ;  SOLID-PHASE EXTRACTION ;  MAGNETIC NANOPARTICLES ;  MONOLITHIC COLUMN ;  GRAPHITE OXIDE ;  LIQUID-CHROMATOGRAPHY ;  GLYCOPEPTIDES ;  CHEMISTRY ;  GLYCOSYLATION
语种: 英语
WOS记录号: WOS:000408288400013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150003
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin Key Lab Biosensing & Mol Recognit, Res Ctr Analyt Sci,Coll Chem, Tianjin 300071, Peoples R China
2.Nankai Univ, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Yang, Jingai,He, Xiwen,Chen, Langxing,et al. Thiol-yne click synthesis of boronic acid functionalized silica nanoparticle-graphene oxide composites for highly selective enrichment of glycoproteins[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1513:118-125.
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