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Thiol-yne click synthesis of boronic acid functionalized silica nanoparticle-graphene oxide composites for highly selective enrichment of glycoproteins
Yang, Jingai1; He, Xiwen1; Chen, Langxing1,2; Zhang, Yukui1,3
KeywordGraphene Oxide Thiol-yne Click Reaction Boronate Affinity Glycoprotein
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2017-09-01
DOI10.1016/j.chroma.2017.07.026
Volume1513Pages:118-125
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordTRANSFER RADICAL POLYMERIZATION ; MOLECULARLY IMPRINTED POLYMERS ; SOLID-PHASE EXTRACTION ; MAGNETIC NANOPARTICLES ; MONOLITHIC COLUMN ; GRAPHITE OXIDE ; LIQUID-CHROMATOGRAPHY ; GLYCOPEPTIDES ; CHEMISTRY ; GLYCOSYLATION
AbstractIn this work, a facile novel strategy is developed for the preparation of 4-mercapto-phenylboronic acid functionalized silica nanoparticle-graphene oxide composite (GO@MPBA) via thiol-yne click reaction. The morphology, structure and composition of the resulting GO@MPBA was characterized by transmission electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, Fourier transform Raman spectroscopy, Thermogravimetric analysis and X-ray photoelectron spectrometry. GO@MPBA exhibited the high binding capacity towards glycoproteins such as ovalbumin (1288.8 mg g(-1)), immunoglobulin G (1144.1 mg g(-1)), transferrin (592.1 mg g(-1)) and horseradish peroxidase (392.4 mg g(-1)), in contrast, the binding capacity of each non-glycoproteins (cytochrome c, deoxyribonuclease A, pepsin, trypsin, lysozyme and bovine serum albumin) is less than 50 mg g-1. Furthermore, the GO@MPBA still remained a good binding capacity after the five times of adsorption-desorption cycles. The selective enrichment of glycoproteins from real egg white samples by GO@MPBA was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. This work could present a facile novel approach of surface modification to design environmentally friendly and more efficient adsorbents for the isolation and enrichment of glycoprotein from complex biological samples. (C) 2017 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000408288400013
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150003
Collection中国科学院大连化学物理研究所
Affiliation1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin Key Lab Biosensing & Mol Recognit, Res Ctr Analyt Sci,Coll Chem, Tianjin 300071, Peoples R China
2.Nankai Univ, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Yang, Jingai,He, Xiwen,Chen, Langxing,et al. Thiol-yne click synthesis of boronic acid functionalized silica nanoparticle-graphene oxide composites for highly selective enrichment of glycoproteins[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1513:118-125.
APA Yang, Jingai,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2017).Thiol-yne click synthesis of boronic acid functionalized silica nanoparticle-graphene oxide composites for highly selective enrichment of glycoproteins.JOURNAL OF CHROMATOGRAPHY A,1513,118-125.
MLA Yang, Jingai,et al."Thiol-yne click synthesis of boronic acid functionalized silica nanoparticle-graphene oxide composites for highly selective enrichment of glycoproteins".JOURNAL OF CHROMATOGRAPHY A 1513(2017):118-125.
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