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Maleic Anhydride Labeling-Based Approach for Quantitative Proteomics and Successive Derivatization of Peptides
Tian, Shanshan1; Zheng, Shuzhen2; Han, Yanpu2; Guo, Zhenchang1; Zhai, Guijin1; Bai, Xue1; He, Xiwen2; Fan, Enguo3,4; Zhang, Yukui2,5; Zhang, Kai1
Source PublicationANALYTICAL CHEMISTRY
2017-08-15
DOI10.1021/acs.analchem.7b01120
Volume89Issue:16Pages:8259-8265
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMASS-SPECTROMETRY ; QUANTIFICATION ; CHEMISTRY ; SILAC ; TAGS ; TOOL
AbstractChemical derivatization is a simple approach for stable-isotope covalent labeling of proteins in quantitative proteomics. Herein we describe the development of a novel maleyl-labeling-based approach for protein quantification. Under optimized conditions, maleic anhydride can serve as a highly efficient reagent to label the amino groups of tryptic peptides. Furthermore, "click chemistry" was successfully applied to obtain the second modification of maleylated peptides via thiol-Michael addition reaction. Accurate quantification was further achieved via the first or/and second step stable-isotope labeling in this study. Our data thus demonstrate that the maleyl-labeling-based method is simple, accurate, and reliable for quantitative proteomics. The developed method not only enables an enhanced sequence coverage of proteins by improving the identification of small and hydrophilic peptides, but also enables a controllable, successive, second derivatization of labeled peptides or proteins, and therefore holds a very promising potential for in-depth analysis of protein structures and dynamics.
Language英语
WOS IDWOS:000407988600013
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150015
Collection中国科学院大连化学物理研究所
Affiliation1.Tianjin Med Univ, Collaborat Innovat Ctr Tianjin Med Epigenet 2011, Minist Educ,Dept Biochem & Mol Biol, Tianjin Key Lab Med Epigenet,Key Lab Immune Micro, Tianjin 300070, Peoples R China
2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
3.Univ Freiburg, Inst Biochem & Mol Biol, Stefan Meier Str 17, D-79104 Freiburg, Germany
4.Chinese Acad Med Sci, Peking Union Med Coll, Sch Basic Med, Dept Microbiol & Parasitol,Inst Basic Med Sci, Beijing 100005, Peoples R China
5.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Liaoning, Peoples R China
Recommended Citation
GB/T 7714
Tian, Shanshan,Zheng, Shuzhen,Han, Yanpu,et al. Maleic Anhydride Labeling-Based Approach for Quantitative Proteomics and Successive Derivatization of Peptides[J]. ANALYTICAL CHEMISTRY,2017,89(16):8259-8265.
APA Tian, Shanshan.,Zheng, Shuzhen.,Han, Yanpu.,Guo, Zhenchang.,Zhai, Guijin.,...&Zhang, Kai.(2017).Maleic Anhydride Labeling-Based Approach for Quantitative Proteomics and Successive Derivatization of Peptides.ANALYTICAL CHEMISTRY,89(16),8259-8265.
MLA Tian, Shanshan,et al."Maleic Anhydride Labeling-Based Approach for Quantitative Proteomics and Successive Derivatization of Peptides".ANALYTICAL CHEMISTRY 89.16(2017):8259-8265.
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