DICP OpenIR
Maleic Anhydride Labeling-Based Approach for Quantitative Proteomics and Successive Derivatization of Peptides
Tian, Shanshan1; Zheng, Shuzhen2; Han, Yanpu2; Guo, Zhenchang1; Zhai, Guijin1; Bai, Xue1; He, Xiwen2; Fan, Enguo3,4; Zhang, Yukui2,5; Zhang, Kai1
刊名ANALYTICAL CHEMISTRY
2017-08-15
DOI10.1021/acs.analchem.7b01120
89期:16页:8259-8265
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MASS-SPECTROMETRY ; QUANTIFICATION ; CHEMISTRY ; SILAC ; TAGS ; TOOL
英文摘要Chemical derivatization is a simple approach for stable-isotope covalent labeling of proteins in quantitative proteomics. Herein we describe the development of a novel maleyl-labeling-based approach for protein quantification. Under optimized conditions, maleic anhydride can serve as a highly efficient reagent to label the amino groups of tryptic peptides. Furthermore, "click chemistry" was successfully applied to obtain the second modification of maleylated peptides via thiol-Michael addition reaction. Accurate quantification was further achieved via the first or/and second step stable-isotope labeling in this study. Our data thus demonstrate that the maleyl-labeling-based method is simple, accurate, and reliable for quantitative proteomics. The developed method not only enables an enhanced sequence coverage of proteins by improving the identification of small and hydrophilic peptides, but also enables a controllable, successive, second derivatization of labeled peptides or proteins, and therefore holds a very promising potential for in-depth analysis of protein structures and dynamics.
语种英语
WOS记录号WOS:000407988600013
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/150015
专题中国科学院大连化学物理研究所
作者单位1.Tianjin Med Univ, Collaborat Innovat Ctr Tianjin Med Epigenet 2011, Minist Educ,Dept Biochem & Mol Biol, Tianjin Key Lab Med Epigenet,Key Lab Immune Micro, Tianjin 300070, Peoples R China
2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
3.Univ Freiburg, Inst Biochem & Mol Biol, Stefan Meier Str 17, D-79104 Freiburg, Germany
4.Chinese Acad Med Sci, Peking Union Med Coll, Sch Basic Med, Dept Microbiol & Parasitol,Inst Basic Med Sci, Beijing 100005, Peoples R China
5.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Liaoning, Peoples R China
推荐引用方式
GB/T 7714
Tian, Shanshan,Zheng, Shuzhen,Han, Yanpu,et al. Maleic Anhydride Labeling-Based Approach for Quantitative Proteomics and Successive Derivatization of Peptides[J]. ANALYTICAL CHEMISTRY,2017,89(16):8259-8265.
APA Tian, Shanshan.,Zheng, Shuzhen.,Han, Yanpu.,Guo, Zhenchang.,Zhai, Guijin.,...&Zhang, Kai.(2017).Maleic Anhydride Labeling-Based Approach for Quantitative Proteomics and Successive Derivatization of Peptides.ANALYTICAL CHEMISTRY,89(16),8259-8265.
MLA Tian, Shanshan,et al."Maleic Anhydride Labeling-Based Approach for Quantitative Proteomics and Successive Derivatization of Peptides".ANALYTICAL CHEMISTRY 89.16(2017):8259-8265.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Tian, Shanshan]的文章
[Zheng, Shuzhen]的文章
[Han, Yanpu]的文章
百度学术
百度学术中相似的文章
[Tian, Shanshan]的文章
[Zheng, Shuzhen]的文章
[Han, Yanpu]的文章
必应学术
必应学术中相似的文章
[Tian, Shanshan]的文章
[Zheng, Shuzhen]的文章
[Han, Yanpu]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。