A hollow fiber system for simple generation of human brain organoids
Zhu, Yujuan1,2,3; Wang, Li1,3; Yin, Fangchao1,2,3; Yu, Yue1,2,3; Wang, Yaqing1,2,3; Liu, Hui1,3; Wang, Hui1,2,3; Sun, Ning4; Liu, Haitao1,2,3; Qin, Jianhua1,2,3,5
Indexed BySCI
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectCell Biology
WOS Research AreaCell Biology
Abstract3D organoids exhibit near-physiological morphogenesis and histology relying on the self-organization of human pluripotent stem cells (hPSCs), representing a new class of in vitro model for studying developmental biology and diseases. An engineered approach is highly desirable to generate sufficient organoids in a simple and efficient manner. Herein, we present a new strategy for the simple formation of massive human brain organoids from hiPSCs within a hollow fiber reactor system by combining fiber materials with the developmental biology principle. A thin and finely adjustable calcium alginate (CaA) core-shell fiber was constructed using a multilayer coaxial laminar flow microfluidic system. The meter-long hollow fibers enabled neural differentiation of hiPSCs and simple formation of abundant brain organoids in a 3D matrix. The generated brain organoids displayed essential features of human brain organogenesis, including polarized neuroepithelium, cell type heterogeneity and discrete brain regions, resembling the early brain development. This approach is simple and easy to operate, which allows for simplified formation of massive brain organoids, overcoming the tedious procedures in conventional methods. In particular, the facile and scalable characteristics of hollow fibers are compatible with real-time observation and monitoring, as well as flexible tissue manipulations for downstream biological analysis. It might also provide a new platform to advance stem cell-derived organoid models and their utility in biomedical applications.
WOS IDWOS:000411014600005
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Document Type期刊论文
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Beijing, Peoples R China
4.Fudan Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Shanghai, Peoples R China
5.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Yujuan,Wang, Li,Yin, Fangchao,et al. A hollow fiber system for simple generation of human brain organoids[J]. INTEGRATIVE BIOLOGY,2017,9(9):774-781.
APA Zhu, Yujuan.,Wang, Li.,Yin, Fangchao.,Yu, Yue.,Wang, Yaqing.,...&Qin, Jianhua.(2017).A hollow fiber system for simple generation of human brain organoids.INTEGRATIVE BIOLOGY,9(9),774-781.
MLA Zhu, Yujuan,et al."A hollow fiber system for simple generation of human brain organoids".INTEGRATIVE BIOLOGY 9.9(2017):774-781.
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