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题名: Metabolic profiling analysis of Siraitia grosvenorii revealed different characteristics of green fruit and saccharified yellow fruit
作者: Fang, Chengnan1, 2;  Wang, Qingqing1, 2;  Liu, Xinyu1, 2;  Xu, Guowang1, 2
关键词: Siraitia grosvenorii ;  UPLC-Q-TOF-MS ;  Metabolic profiling ;  Saccharification ;  Distribution characteristics
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2017-10-25
DOI: 10.1016/j.jpba.2017.06.046
卷: 145, 页:158-168
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ;  Pharmacology & Pharmacy
英文摘要: Siraitia grosvenorii is an economic and medicinal plant, its fruit is considered to be good to health for its diverse bioactive ingredients. However, the clarification of chemical composition and their changes after saccharification procedure are not well performed. In present study, a metabolomics method based on ultra-high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry was developed for metabolic profiling acquisition ofSiraitia grosvenorii extract. Furthermore, information dependent analysis (IDA) combined with self-constructed LC MS/MS identification system for metabolites were employed to identify primary and secondary metabolites in Siraitia grosvenorii. A total of 126 metabolites were identified or tentatively identified. The obvious differences of metabolic profiling between green fruit and saccharified yellow fruit were observed, and metabolites showed their own distribution characteristics in peel, flesh and seed. The majority of the nutrients and effective components were more distributed in flesh and peel, and saccharification was conducive to accumulation of sweet glycosides. This study not only expanded metabolite composition information of Siraitia grosvenorii, but also specified distribution characteristics of identified metabolites. (C) 2017 Elsevier B.V. All rights reserved.
关键词[WOS]: FLIGHT MASS-SPECTROMETRY ;  LUO-HAN-GUO ;  LIQUID-CHROMATOGRAPHY ;  COMPREHENSIVE ANALYSIS ;  CHEMICAL-CONSTITUENTS ;  MOGROSIDE V ;  IN-VITRO ;  GLYCOSIDES ;  TRITERPENE ;  IDENTIFICATION
语种: 英语
WOS记录号: WOS:000410872200021
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150039
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Fang, Chengnan,Wang, Qingqing,Liu, Xinyu,et al. Metabolic profiling analysis of Siraitia grosvenorii revealed different characteristics of green fruit and saccharified yellow fruit[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2017,145:158-168.
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