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题名: ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo
作者: Shi, Min1;  Zhang, Heng1;  Wu, Xudong2;  He, Zhisong3;  Wang, Lantian1;  Yin, Shanye4;  Tian, Bin5;  Li, Guohui2;  Cheng, Hong1
刊名: NUCLEIC ACIDS RESEARCH
发表日期: 2017-09-19
DOI: 10.1093/nar/gkx597
卷: 45, 期:16, 页:9640-9653
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: The TREX complex (TREX) plays key roles in nuclear export of mRNAs. However, little is known about its transcriptome-wide binding targets. We used individual cross-linking and immunoprecipitation (iCLIP) to identify the binding sites of ALYREF, an mRNA export adaptor in TREX, in human cells. Consistent with previous in vitro studies, ALYREF binds to a region near the 5' end of the mRNA in a CBP80-dependent manner. Unexpectedly, we identified PABPN1-dependent ALYREF binding near the 3' end of the mRNA. Furthermore, the 3' processing factor CstF64 directly interacts with ALYREF and is required for the overall binding of ALYREF on the mRNA. In addition, we found that numerous middle exons harbor ALYREF binding sites and identified ALYREF-binding motifs that promote nuclear export of intronless mRNAs. Together, our study defines enrichment of ALYREF binding sites at the 5' and the 3' regions of the mRNA in vivo, identifies export-promoting ALYREF-binding motifs, and reveals CstF64-and PABPN1-mediated coupling of mRNA nuclear export to 3' processing.
关键词[WOS]: HUMAN TREX COMPLEX ;  GENE-EXPRESSION ;  NUCLEAR EXPORT ;  COTRANSCRIPTIONAL RECRUITMENT ;  SPLICING PROMOTES ;  MRNP FORMATION ;  THO COMPLEX ;  INTRONLESS ;  ELEMENT ;  TRANSPORT
语种: 英语
WOS记录号: WOS:000411096400037
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150044
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai 200031, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Mol Modeling & Design, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, CAS Key Lab Computat Biol, Shanghai 200031, Peoples R China
4.Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USA
5.Rutgers New Jersey Med Sch, Dept Microbiol Biochem & Mol Genet, Newark, NJ 07103 USA

Recommended Citation:
Shi, Min,Zhang, Heng,Wu, Xudong,et al. ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo[J]. NUCLEIC ACIDS RESEARCH,2017,45(16):9640-9653.
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