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ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo
Shi, Min1; Zhang, Heng1; Wu, Xudong2; He, Zhisong3; Wang, Lantian1; Yin, Shanye4; Tian, Bin5; Li, Guohui2; Cheng, Hong1
刊名NUCLEIC ACIDS RESEARCH
2017-09-19
DOI10.1093/nar/gkx597
45期:16页:9640-9653
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]HUMAN TREX COMPLEX ; GENE-EXPRESSION ; NUCLEAR EXPORT ; COTRANSCRIPTIONAL RECRUITMENT ; SPLICING PROMOTES ; MRNP FORMATION ; THO COMPLEX ; INTRONLESS ; ELEMENT ; TRANSPORT
英文摘要The TREX complex (TREX) plays key roles in nuclear export of mRNAs. However, little is known about its transcriptome-wide binding targets. We used individual cross-linking and immunoprecipitation (iCLIP) to identify the binding sites of ALYREF, an mRNA export adaptor in TREX, in human cells. Consistent with previous in vitro studies, ALYREF binds to a region near the 5' end of the mRNA in a CBP80-dependent manner. Unexpectedly, we identified PABPN1-dependent ALYREF binding near the 3' end of the mRNA. Furthermore, the 3' processing factor CstF64 directly interacts with ALYREF and is required for the overall binding of ALYREF on the mRNA. In addition, we found that numerous middle exons harbor ALYREF binding sites and identified ALYREF-binding motifs that promote nuclear export of intronless mRNAs. Together, our study defines enrichment of ALYREF binding sites at the 5' and the 3' regions of the mRNA in vivo, identifies export-promoting ALYREF-binding motifs, and reveals CstF64-and PABPN1-mediated coupling of mRNA nuclear export to 3' processing.
语种英语
WOS记录号WOS:000411096400037
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/150044
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai 200031, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Mol Modeling & Design, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, CAS Key Lab Computat Biol, Shanghai 200031, Peoples R China
4.Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USA
5.Rutgers New Jersey Med Sch, Dept Microbiol Biochem & Mol Genet, Newark, NJ 07103 USA
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Shi, Min,Zhang, Heng,Wu, Xudong,et al. ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo[J]. NUCLEIC ACIDS RESEARCH,2017,45(16):9640-9653.
APA Shi, Min.,Zhang, Heng.,Wu, Xudong.,He, Zhisong.,Wang, Lantian.,...&Cheng, Hong.(2017).ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo.NUCLEIC ACIDS RESEARCH,45(16),9640-9653.
MLA Shi, Min,et al."ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo".NUCLEIC ACIDS RESEARCH 45.16(2017):9640-9653.
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