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Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS
Wang, Qi1,2; Wang, Keyun1,2; Ye, Mingliang1,2
刊名ANALYST
2017-10-17
DOI10.1039/c7an00954b
142期:19页:3536-3548
收录类别SCI
文章类型Review
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]ADENOSYL-L-METHIONINE ; AFFINITY-CHROMATOGRAPHY ADSORBENT ; POSITIVE-ION MODE ; MASS-SPECTROMETRY ; LYSINE-METHYLATION ; ARGININE METHYLATION ; PROTEOMIC ANALYSIS ; POSTTRANSLATIONAL MODIFICATIONS ; HUMAN-CELLS ; IN-VIVO
英文摘要Protein methylation is an important post-translational modification (PTM) that plays crucial roles in the regulation of diverse biological processes. Though many efforts have been devoted to the investigation of protein methylation, the analysis of non-histone methylation at the proteome level is still a great challenge. The alteration of the protein/peptide physicochemical properties caused by methylation is very small, thus it is difficult to develop highly efficient enrichment approaches to separate methylated peptides from a pool of diverse background peptides. The mass shifts caused by methylations are identical to the substitutions of some amino acids, thus it is difficult to confidently identify methylated peptides. In this review, we report on recent advances in the development of methods for large-scale analysis of nonhistone protein methylation. Especially the methods for efficient enrichment and the approaches for controlling identification confidence have been covered.
语种英语
WOS记录号WOS:000411703800001
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/150048
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Wang, Qi,Wang, Keyun,Ye, Mingliang. Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS[J]. ANALYST,2017,142(19):3536-3548.
APA Wang, Qi,Wang, Keyun,&Ye, Mingliang.(2017).Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS.ANALYST,142(19),3536-3548.
MLA Wang, Qi,et al."Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS".ANALYST 142.19(2017):3536-3548.
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