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Preparation and optimization of calcium pectate beads for cell encapsulation
Zhao, Shan1,2,3; Zhang, Ying2; Liu, Yang2; Yang, Fan4; Xiu, Zhilong1; Ma, Xiaojun4; Sun, Guangwei2
KeywordBiomaterials Biomedical Applications Gels
Source PublicationJOURNAL OF APPLIED POLYMER SCIENCE
2017-10-29
DOI10.1002/app.45685
Volume135Issue:2
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectPolymer Science
WOS Research AreaPolymer Science
WOS KeywordLOW-METHOXYL-PECTIN ; EGG-BOX MODEL ; GEL BEADS ; BIOMEDICAL APPLICATIONS ; CA2+-PECTIN GELS ; DIVALENT-CATIONS ; DRUG-DELIVERY ; CITRUS PECTIN ; FT-IR ; ALGINATE
AbstractA variety of natural polymers are considered suitable for cell entrapment. Pectin, a natural polysaccharide found in the cell walls of all higher terrestrial plants, presents great potential for cell encapsulation because of its ability to form gels. In this study, we investigated the influences of intrinsic and extrinsic factors on the formation and properties of calcium pectate (CP). Beads of CP prepared with 30-35% methyl esterification presented improved gel properties. The molecular weight (M-w), calcium content, and gel time did not significantly affect the gel properties. The gelling properties, sphericity factor (SF), and swelling ratio were significantly influenced by the degree of methyl esterification (DE) and pectin concentration. The DE (<40%), M-w, and calcium concentration had negative effects on the SFs of CP beads. Moreover, increases in the DE (<40%) and pectin concentration increased the swelling ratio. The C3A cells encapsulated in optimized CP beads maintained good viability and proliferation for up to 2 weeks. In conclusion, CP beads are a potential encapsulation material for cell immobilization and application in related fields. (c) 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 45685.
Language英语
WOS IDWOS:000411806400024
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150049
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Sci Res Ctr Translat Med, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Shan,Zhang, Ying,Liu, Yang,et al. Preparation and optimization of calcium pectate beads for cell encapsulation[J]. JOURNAL OF APPLIED POLYMER SCIENCE,2017,135(2).
APA Zhao, Shan.,Zhang, Ying.,Liu, Yang.,Yang, Fan.,Xiu, Zhilong.,...&Sun, Guangwei.(2017).Preparation and optimization of calcium pectate beads for cell encapsulation.JOURNAL OF APPLIED POLYMER SCIENCE,135(2).
MLA Zhao, Shan,et al."Preparation and optimization of calcium pectate beads for cell encapsulation".JOURNAL OF APPLIED POLYMER SCIENCE 135.2(2017).
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