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Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis
Liu, Lukuan1,2,3; Yang, Kaiguang1; Zhu, Xudong1,2; Liang, Yu1; Chen, Yuanbo1,2; Fang, Fei1,2; Zhao, Qun1; Zhang, Lihua1; Zhang, Yukui1
KeywordCirculating Tumor Cells Aptamer Proteome Open Tubular Column
Source PublicationTALANTA
2017-12-01
DOI10.1016/j.talanta.2017.07.041
Volume175Pages:189-193
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMULTIPLE TYPES ; NANOPARTICLES ; ENRICHMENT ; CANCER ; ATTACHMENT ; CHALLENGES ; SEPARATION ; SURFACES ; POLYMER
AbstractCirculating tumor cells hold the key to predicting the prognosis and discovering the therapeutic targets. Herein, we proposed a strategy to develop an aptamer-immobilized open tubular capillary column by which SMMC-7721 human hepatoma cells (SMMC-7721 cells) could be captured with an over 70% of capture efficiency and a 3.0 +/- 0.2 of enrichment factor. Owing to the compatibility of the column, the captured cells by the column could be analyzed by LC-MS from protein level and 5 unique proteins of SMMC-7721 cells were identified which could be used as markers to identify SMMC-7721 cells when Jurkat T-leukemia cells (Jurkat cells) were employed as interfering cells. As the key component, the aptamer-immobilized column had the potential to be integrated into the platform for separating, enriching and characterizing rare cells simultaneously.
Language英语
WOS IDWOS:000412033100024
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150063
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
Recommended Citation
GB/T 7714
Liu, Lukuan,Yang, Kaiguang,Zhu, Xudong,et al. Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis[J]. TALANTA,2017,175:189-193.
APA Liu, Lukuan.,Yang, Kaiguang.,Zhu, Xudong.,Liang, Yu.,Chen, Yuanbo.,...&Zhang, Yukui.(2017).Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis.TALANTA,175,189-193.
MLA Liu, Lukuan,et al."Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis".TALANTA 175(2017):189-193.
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