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Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe
Jin, Qiang1,2,3,5; Feng, Lei4,6; Zhang, Shui-Jun1,5; Wang, Dan-Dan2; Wang, Fang-Jun3; Zhang, Yi1; Cui, Jing-Nan4; Guo, Wen-Zhi1; Ge, Guang-Bo2,3; Yang, Ling2
刊名ANALYTICAL CHEMISTRY
2017-09-19
DOI10.1021/acs.analchem.7b01975
89期:18页:9884-9891
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]HUMAN SERUM-ALBUMIN ; PSEUDO-ESTERASE ACTIVITY ; HUMAN CARBOXYLESTERASE 1 ; OFF-ON PROBE ; IN-VIVO ; ENZYMATIC-PROPERTIES ; BROMCRESOL PURPLE ; PRACTICAL ASPECTS ; BINDING ; NITROREDUCTASE
英文摘要In this study, a novel fluorescent detection system for biological sensing of human albumin (HA) was developed on the basis of the pseudoesterase activity and substrate preference of HA. The designed near-infrared (NIR) fluorescent probe (DDAP) could be effectively hydrolyzed by HA, accompanied by significant changes in both color and fluorescence spectrum. The sensing mechanism was fully investigated by fluorescence spectroscopy, NMR, and mass spectra. DDAP exhibited excellent selectivity and sensitivity toward HA over a variety of human plasma proteins, hydrolases, and abundant biomolecules found in human body. The probe has been successfully applied to measure native HA in diluted plasma samples and the secreted HA in the hepatocyte culture supernatant. DDAP has also been used for fluorescence imaging of HA reabsorption in living renal cells, and the results show that the probe exhibits good cell permeability, low cytotoxicity and high imaging resolution. Furthermore, DDAP has been successfully used for real-time tracking the uptaking and degradation of albumin in ex vivo mouse kidney models for the first time. All these results clearly demonstrated that DDAP-based assay held great promise for real-time sensing and tracking HA in complex biological systems, which would be very useful for basic researches and clinical diagnosis of HA associated diseases.
语种英语
WOS记录号WOS:000411549100040
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/150069
专题中国科学院大连化学物理研究所
作者单位1.Zhengzhou Univ, Dept Hepatobiliary & Pancreat Surg, Affiliated Hosp 1, Zhengzhou 450001, Henan, Peoples R China
2.Shanghai Univ Tradit Chinese Med, Inst Interdisciplinary Med, Shanghai 201213, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
4.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
5.Zhengzhou Univ, Key Lab Hepatobiliary & Pancreat Surg & Digest Or, Affiliated Hosp 1, Zhengzhou 450001, Henan, Peoples R China
6.Dalian Med Univ, Acad Integrat Med, Coll Pharm, Dalian 116044, Peoples R China
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GB/T 7714
Jin, Qiang,Feng, Lei,Zhang, Shui-Jun,et al. Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe[J]. ANALYTICAL CHEMISTRY,2017,89(18):9884-9891.
APA Jin, Qiang.,Feng, Lei.,Zhang, Shui-Jun.,Wang, Dan-Dan.,Wang, Fang-Jun.,...&Yang, Ling.(2017).Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe.ANALYTICAL CHEMISTRY,89(18),9884-9891.
MLA Jin, Qiang,et al."Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe".ANALYTICAL CHEMISTRY 89.18(2017):9884-9891.
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