中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe
作者: Jin, Qiang1, 2, 3, 5;  Feng, Lei4, 6;  Zhang, Shui-Jun1, 5;  Wang, Dan-Dan2;  Wang, Fang-Jun3;  Zhang, Yi1;  Cui, Jing-Nan4;  Guo, Wen-Zhi1;  Ge, Guang-Bo2, 3;  Yang, Ling2
刊名: ANALYTICAL CHEMISTRY
发表日期: 2017-09-19
DOI: 10.1021/acs.analchem.7b01975
卷: 89, 期:18, 页:9884-9891
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: In this study, a novel fluorescent detection system for biological sensing of human albumin (HA) was developed on the basis of the pseudoesterase activity and substrate preference of HA. The designed near-infrared (NIR) fluorescent probe (DDAP) could be effectively hydrolyzed by HA, accompanied by significant changes in both color and fluorescence spectrum. The sensing mechanism was fully investigated by fluorescence spectroscopy, NMR, and mass spectra. DDAP exhibited excellent selectivity and sensitivity toward HA over a variety of human plasma proteins, hydrolases, and abundant biomolecules found in human body. The probe has been successfully applied to measure native HA in diluted plasma samples and the secreted HA in the hepatocyte culture supernatant. DDAP has also been used for fluorescence imaging of HA reabsorption in living renal cells, and the results show that the probe exhibits good cell permeability, low cytotoxicity and high imaging resolution. Furthermore, DDAP has been successfully used for real-time tracking the uptaking and degradation of albumin in ex vivo mouse kidney models for the first time. All these results clearly demonstrated that DDAP-based assay held great promise for real-time sensing and tracking HA in complex biological systems, which would be very useful for basic researches and clinical diagnosis of HA associated diseases.
关键词[WOS]: HUMAN SERUM-ALBUMIN ;  PSEUDO-ESTERASE ACTIVITY ;  HUMAN CARBOXYLESTERASE 1 ;  OFF-ON PROBE ;  IN-VIVO ;  ENZYMATIC-PROPERTIES ;  BROMCRESOL PURPLE ;  PRACTICAL ASPECTS ;  BINDING ;  NITROREDUCTASE
语种: 英语
WOS记录号: WOS:000411549100040
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150069
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Zhengzhou Univ, Dept Hepatobiliary & Pancreat Surg, Affiliated Hosp 1, Zhengzhou 450001, Henan, Peoples R China
2.Shanghai Univ Tradit Chinese Med, Inst Interdisciplinary Med, Shanghai 201213, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
4.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
5.Zhengzhou Univ, Key Lab Hepatobiliary & Pancreat Surg & Digest Or, Affiliated Hosp 1, Zhengzhou 450001, Henan, Peoples R China
6.Dalian Med Univ, Acad Integrat Med, Coll Pharm, Dalian 116044, Peoples R China

Recommended Citation:
Jin, Qiang,Feng, Lei,Zhang, Shui-Jun,et al. Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe[J]. ANALYTICAL CHEMISTRY,2017,89(18):9884-9891.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Jin, Qiang]'s Articles
 [Feng, Lei]'s Articles
 [Zhang, Shui-Jun]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Jin, Qiang]‘s Articles
 [Feng, Lei]‘s Articles
 [Zhang, Shui-Jun]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace