DICP OpenIR
Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe
Jin, Qiang1,2,3,5; Feng, Lei4,6; Zhang, Shui-Jun1,5; Wang, Dan-Dan2; Wang, Fang-Jun3; Zhang, Yi1; Cui, Jing-Nan4; Guo, Wen-Zhi1; Ge, Guang-Bo2,3; Yang, Ling2
Source PublicationANALYTICAL CHEMISTRY
2017-09-19
DOI10.1021/acs.analchem.7b01975
Volume89Issue:18Pages:9884-9891
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordHUMAN SERUM-ALBUMIN ; PSEUDO-ESTERASE ACTIVITY ; HUMAN CARBOXYLESTERASE 1 ; OFF-ON PROBE ; IN-VIVO ; ENZYMATIC-PROPERTIES ; BROMCRESOL PURPLE ; PRACTICAL ASPECTS ; BINDING ; NITROREDUCTASE
AbstractIn this study, a novel fluorescent detection system for biological sensing of human albumin (HA) was developed on the basis of the pseudoesterase activity and substrate preference of HA. The designed near-infrared (NIR) fluorescent probe (DDAP) could be effectively hydrolyzed by HA, accompanied by significant changes in both color and fluorescence spectrum. The sensing mechanism was fully investigated by fluorescence spectroscopy, NMR, and mass spectra. DDAP exhibited excellent selectivity and sensitivity toward HA over a variety of human plasma proteins, hydrolases, and abundant biomolecules found in human body. The probe has been successfully applied to measure native HA in diluted plasma samples and the secreted HA in the hepatocyte culture supernatant. DDAP has also been used for fluorescence imaging of HA reabsorption in living renal cells, and the results show that the probe exhibits good cell permeability, low cytotoxicity and high imaging resolution. Furthermore, DDAP has been successfully used for real-time tracking the uptaking and degradation of albumin in ex vivo mouse kidney models for the first time. All these results clearly demonstrated that DDAP-based assay held great promise for real-time sensing and tracking HA in complex biological systems, which would be very useful for basic researches and clinical diagnosis of HA associated diseases.
Language英语
WOS IDWOS:000411549100040
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150069
Collection中国科学院大连化学物理研究所
Affiliation1.Zhengzhou Univ, Dept Hepatobiliary & Pancreat Surg, Affiliated Hosp 1, Zhengzhou 450001, Henan, Peoples R China
2.Shanghai Univ Tradit Chinese Med, Inst Interdisciplinary Med, Shanghai 201213, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
4.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
5.Zhengzhou Univ, Key Lab Hepatobiliary & Pancreat Surg & Digest Or, Affiliated Hosp 1, Zhengzhou 450001, Henan, Peoples R China
6.Dalian Med Univ, Acad Integrat Med, Coll Pharm, Dalian 116044, Peoples R China
Recommended Citation
GB/T 7714
Jin, Qiang,Feng, Lei,Zhang, Shui-Jun,et al. Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe[J]. ANALYTICAL CHEMISTRY,2017,89(18):9884-9891.
APA Jin, Qiang.,Feng, Lei.,Zhang, Shui-Jun.,Wang, Dan-Dan.,Wang, Fang-Jun.,...&Yang, Ling.(2017).Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe.ANALYTICAL CHEMISTRY,89(18),9884-9891.
MLA Jin, Qiang,et al."Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe".ANALYTICAL CHEMISTRY 89.18(2017):9884-9891.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jin, Qiang]'s Articles
[Feng, Lei]'s Articles
[Zhang, Shui-Jun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jin, Qiang]'s Articles
[Feng, Lei]'s Articles
[Zhang, Shui-Jun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jin, Qiang]'s Articles
[Feng, Lei]'s Articles
[Zhang, Shui-Jun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.