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题名: High-throughput Screening of Potential Allergens from Complex Proteins of Large Yellow Croaker (Larimichthys crocea) by Mass Spectrometry
作者: Yu Yang1, 2;  Gao Qing-Li1;  Song Yang1;  Zhang Jing-Yu1;  Li Zhi-Bo1;  Li Wei1;  Jin Yan2;  Li Meng1;  Zhao Qian-Cheng1;  Qi Yan-Xia1
关键词: Larimichthys crocea ;  Allergens ;  Mass spectrometry ;  Sequence homology analysis ;  Simulated gastric fluid digestion
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2017-05-01
DOI: 10.1016/S1872-2040(17)61016-8
卷: 45, 期:5, 页:1713-1718
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The allergenicity of large yellow croaker (Larimichthys crocea) proteins has been widely reported to cause many serious food safety problems. In this study, the protein sample extracted from Larimichthys crocea was digested and analyzed by reversed phase liquid chromatography coupled with tandem mass spectrometry (RPLC-MS/MS) for globally proteomic analysis, and the Larimichthys crocea proteins were digested with simulated gastric fluid (SGF) at different time intervals and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The digestion-resistant proteins were further done with in-gel digestion and analyzed by RPLC-MS/MS. Then, sequence homology analysis between identified proteins and known allergens was performed. Results showed that 33 kinds of distinct proteins were feasibly identified from Larimichthys crocea by RPLC-MS/MS and the protein band (approximately 44.0 kDa) presented in SDS-PAGE was considered as protein-resistant to SGF digestion. Two potential allergens, beta-enolase and tropomyosin alpha-1 chain, were found from proteins resistant to SGF digestion by sequence homology analysis. It was observed that most of these SGF digestion-resistant proteins were potential allergens with high sequences similarity to the known protein allergens from other species. Therefore, this strategy provides an efficient alternative for potential allergens screening with high-throughput, which exhibits great potential application in fish protein allergens related food safety control.
关键词[WOS]: FOOD ALLERGENS ;  LIQUID-CHROMATOGRAPHY ;  FRAGRANCE ALLERGENS ;  IN-VITRO ;  DIGESTION ;  FISH ;  PARVALBUMIN ;  STABILITY ;  SHOTGUN ;  WATER
语种: 英语
WOS记录号: WOS:000412224800001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150083
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Ocean Univ, Coll Food Sci & Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Yu Yang,Gao Qing-Li,Song Yang,et al. High-throughput Screening of Potential Allergens from Complex Proteins of Large Yellow Croaker (Larimichthys crocea) by Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2017,45(5):1713-1718.
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