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MicroRNA-205-5p regulates the chemotherapeutic resistance of hepatocellular carcinoma cells by targeting PTEN/JNK/ANXA3 pathway
Shao, Ping1; Qu, Wei-Kun1; Wang, Cheng-Ye1; Tian, Yu1; Ye, Ming-Liang2; Sun, De-Guang1; Sui, Ji-Dong1; Wang, Li-Ming1; Fan, Rong3; Gao, Zhen-Ming1
关键词Microrna-205 Hepatocellular Carcinoma Chemotherapeutic Resistance Pten
刊名AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
2017
9期:9页:4300-+
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Oncology ; Medicine, Research & Experimental
研究领域[WOS]Oncology ; Research & Experimental Medicine
关键词[WOS]COLORECTAL-CANCER CELLS ; TUMOR-SUPPRESSOR ; MIR-205 ; PTEN ; PROLIFERATION ; EXPRESSION ; PROMOTES ; PROTEIN ; GROWTH ; CHEMORESISTANCE
英文摘要Hepatocellular carcinoma (HCC) is a common malignant tumor of the digestive system, and patients with advanced HCC have a poor outlook, partly due to resistance to chemotherapeutic drugs. Previous studies have implicated microRNAs in the regulation of chemoresistance, and we have previously shown that microRNA (miR)-205- 5p is down-regulated in multiple hepatoma cell lines. Here, we investigate whether miR-205-5p is involved in chemotherapeutic resistance in HCC. Expression of miR-205-5p was measured by real-time quantitative reverse transcription PCR and cell viability was determined using a CCK-8 cell viability assay. Expression of proteins in the PTEN/JNK/ANXA3 pathway were assessed via Western blotting. We found that miR-205-5p expression was down-regulated in all HCC cell lines investigated. In addition, miR-205-5p expression was upregulated by 5-fluorouracil (5-Fu) treatment in Bel-7402 (Bel) cells. Interestingly, miR-205-5p expression was increased in multidrug-resistant Bel-7402/5-Fu (Bel/Fu) cells, compared with Bel cells. We next demonstrated that sensitivity to 5-Fu was increased in Bel/Fu cells after treatment with a miR-205-5p inhibitor. Similarly, increased resistance to 5-Fu was observed in Bel cells after transfection with a miR-205-5p mimic. We injected nude mice with Bel/5-Fu cells to promote tumor growth, and found that co-treatment with a miR-205-5p antagomir and 5-Fu slowed tumor growth more than either treatment alone. Finally, we found that these effects were all associated with changes in the PTEN/JNK/ANXA3 pathway. In conclusion, inhibition of miR-205-5p may reverse chemotherapeutic resistance to 5-Fu, and this may occur via the PTEN/JNK/ANXA3 pathway.
语种英语
WOS记录号WOS:000412145400038
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/150104
专题中国科学院大连化学物理研究所
作者单位1.Dalian Med Univ, Affiliated Hosp 2, Dept Surg, Div Hepatobiliary & Pancreat Surg, 467 Zhongshan Rd, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, VIP Ward 2, 467 Zhongshan Rd, Dalian 116023, Peoples R China
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Shao, Ping,Qu, Wei-Kun,Wang, Cheng-Ye,et al. MicroRNA-205-5p regulates the chemotherapeutic resistance of hepatocellular carcinoma cells by targeting PTEN/JNK/ANXA3 pathway[J]. AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,2017,9(9):4300-+.
APA Shao, Ping.,Qu, Wei-Kun.,Wang, Cheng-Ye.,Tian, Yu.,Ye, Ming-Liang.,...&Gao, Zhen-Ming.(2017).MicroRNA-205-5p regulates the chemotherapeutic resistance of hepatocellular carcinoma cells by targeting PTEN/JNK/ANXA3 pathway.AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,9(9),4300-+.
MLA Shao, Ping,et al."MicroRNA-205-5p regulates the chemotherapeutic resistance of hepatocellular carcinoma cells by targeting PTEN/JNK/ANXA3 pathway".AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH 9.9(2017):4300-+.
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