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A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate
Qiao, Qinglong1,2; Liu, Wenjuan2; Chen, Jie2; Zhou, Wei1,2; Yin, Wenting2; Miao, Lu2; Cui, Jingnan1; Xu, Zhaochao2
关键词Fluorogenic Probe Snap-tag Labeling Rate Protein Imaging Wash Free
刊名DYES AND PIGMENTS
2017-12-01
DOI10.1016/j.dyepig.2017.08.032
147页:327-333
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Applied ; Engineering, Chemical ; Materials Science, Textiles
研究领域[WOS]Chemistry ; Engineering ; Materials Science
关键词[WOS]LIVING CELLS ; FLUORESCENT-PROBE ; PROTEIN ; FLUOROPHORE
英文摘要SNAP-tag is one of most popular genetically encoded protein tags that can be labeled with fluorescent molecules for visualizing a protein of interest in live cells. Fluorogenic probes keep dark until they label protein tags, significantly improving the signal-to-noise ratio to image proteins without wash-out step. However, most of reported fluorogenic probes for SNAP-tag suffered from the low or mild labeling rate comparing with non-fluorogenic ones. In this paper, we reported a 4-amino-naphthliamide derived fluorogenic probe for SNAP-tag, which exhibited the fast record labeling rate among fluorogenic probes. Finally, we applied this probe to image proteins in mitochondria and nucleus in live cells without washout steps. (C) 2017 Elsevier Ltd. All rights reserved.
语种英语
WOS记录号WOS:000412612400037
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/150105
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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Qiao, Qinglong,Liu, Wenjuan,Chen, Jie,et al. A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate[J]. DYES AND PIGMENTS,2017,147:327-333.
APA Qiao, Qinglong.,Liu, Wenjuan.,Chen, Jie.,Zhou, Wei.,Yin, Wenting.,...&Xu, Zhaochao.(2017).A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate.DYES AND PIGMENTS,147,327-333.
MLA Qiao, Qinglong,et al."A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate".DYES AND PIGMENTS 147(2017):327-333.
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