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Deletion of acetate transporter gene ADY2 improved tolerance of Saccharomyces cerevisiae against multiple stresses and enhanced ethanol production in the presence of acetic acid
Zhang, Mingming3; Zhang, Keyu1,2; Mehmood, Muhammad Aamer1,2,5; Zhao, Zongbao Kent4; Bai, Fengwu1,2,3; Zhao, Xinqing1,2
KeywordSaccharomyces Cerevisiae Acetic Acid Stress Tolerance Zinc Sulfate Ady2p Ethanol Production
Source PublicationBIORESOURCE TECHNOLOGY
2017-12-01
DOI10.1016/j.biortech.2017.05.191
Volume245Pages:1461-1468
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS KeywordSELF-FLOCCULATING YEAST ; PROGRAMMED CELL-DEATH ; TRANSCRIPTOME ANALYSIS ; LABORATORY EVOLUTION ; IDENTIFICATION ; ZINC ; OVEREXPRESSION ; FERMENTATION ; RESISTANCE ; SUPPLEMENTATION
AbstractThe aim of this work was to study the effects of deleting acetate transporter gene ADY2 on growth and fermentation of Saccharomyces cerevisiae in the presence of inhibitors. Comparative transcriptome analysis revealed that three genes encoding plasma membrane carboxylic acid transporters, especially ADY2, were significantly downregulated under the zinc sulfate addition condition in the presence of acetic acid stress, and the deletion of ADY2 improved growth of S. cerevisiae under acetic acid, ethanol and hydrogen peroxide stresses. Consistently, a concomitant increase in ethanol production by 14.7% in the presence of 3.6 g/L acetic acid was observed in the ADY2 deletion mutant of S. cerevisiae BY4741. Decreased intracellular acetic acid, ROS accumulation, and plasma membrane permeability were observed in the ADY2 deletion mutant. These findings would be useful for developing robust yeast strains for efficient ethanol production. (C) 2017 Elsevier Ltd. All rights reserved.
Language英语
WOS IDWOS:000413038300021
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150123
Collection中国科学院大连化学物理研究所
Affiliation1.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
2.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China
3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
5.Govt Coll Univ Faisalabad, Dept Bioinformat & Biotechnol, Bioenergy Res Ctr, Faisalabad 38000, Pakistan
Recommended Citation
GB/T 7714
Zhang, Mingming,Zhang, Keyu,Mehmood, Muhammad Aamer,et al. Deletion of acetate transporter gene ADY2 improved tolerance of Saccharomyces cerevisiae against multiple stresses and enhanced ethanol production in the presence of acetic acid[J]. BIORESOURCE TECHNOLOGY,2017,245:1461-1468.
APA Zhang, Mingming,Zhang, Keyu,Mehmood, Muhammad Aamer,Zhao, Zongbao Kent,Bai, Fengwu,&Zhao, Xinqing.(2017).Deletion of acetate transporter gene ADY2 improved tolerance of Saccharomyces cerevisiae against multiple stresses and enhanced ethanol production in the presence of acetic acid.BIORESOURCE TECHNOLOGY,245,1461-1468.
MLA Zhang, Mingming,et al."Deletion of acetate transporter gene ADY2 improved tolerance of Saccharomyces cerevisiae against multiple stresses and enhanced ethanol production in the presence of acetic acid".BIORESOURCE TECHNOLOGY 245(2017):1461-1468.
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