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题名: Enhancing the Mass Spectrometry Sensitivity for Oligonucleotide Detection by Organic Vapor Assisted Electrospray
作者: Weng, Guofeng1, 2;  Liu, Zheyi2, 3;  Chen, Jin2, 3;  Wang, Fangjun2;  Pan, Yuanjiang1;  Zhang, Yukui2
刊名: ANALYTICAL CHEMISTRY
发表日期: 2017-10-03
DOI: 10.1021/acs.analchem.7b01695
卷: 89, 期:19, 页:10256-10263
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: There are two challenges in oligonucleotide detection by liquid chromatography coupled with mass spectrometry (LC-MS), the serious ion suppression effects caused by ion-pair reagents and the low detection sensitivity in positive mode MS. In this study, highly concentrated alcohol vapors were introduced into an enclosed electrospray ionization chamber, and oligonucleotides could be well detected in negative mode MS even with 100 mM triethylammonium acetate (TEAA) as an ion-pair reagent. The MS signal intensity was improved 600-fold (for standard oligonucleotide dT15) by the isopropanol vapor assisted electrospray, and effective ion-pair LC separation was feasibly coupled with high-sensitive MS detection. Then, oligonucleotides were successfully detected in positive mode MS with few adducts by propanoic acid vapor assisted electrospray. The signal intensity was enhanced more than 10-fold on average compared with adding acids into the electrospray solution. Finally, oligonucleotides and peptides or histones were simultaneously detected in MS with little interference with each other. Our strategy provides a useful alternative for investigating the biological functions of oligonucleotides.
关键词[WOS]: CHEMICALLY-MODIFIED OLIGONUCLEOTIDES ;  COLLISION-INDUCED DISSOCIATION ;  LIQUID-CHROMATOGRAPHY ;  NUCLEIC-ACIDS ;  POSTTRANSLATIONAL MODIFICATIONS ;  DNA OLIGONUCLEOTIDES ;  PROTEOME ANALYSIS ;  CHARGED DROPLETS ;  CATION-EXCHANGE ;  IONIZATION
语种: 英语
WOS记录号: WOS:000412716800023
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150127
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Zhejiang Univ, Dept Chem, Hangzhou 310027, Zhejiang, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Weng, Guofeng,Liu, Zheyi,Chen, Jin,et al. Enhancing the Mass Spectrometry Sensitivity for Oligonucleotide Detection by Organic Vapor Assisted Electrospray[J]. ANALYTICAL CHEMISTRY,2017,89(19):10256-10263.
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