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Enhancing the Mass Spectrometry Sensitivity for Oligonucleotide Detection by Organic Vapor Assisted Electrospray
Weng, Guofeng1,2; Liu, Zheyi2,3; Chen, Jin2,3; Wang, Fangjun2; Pan, Yuanjiang1; Zhang, Yukui2
刊名ANALYTICAL CHEMISTRY
2017-10-03
DOI10.1021/acs.analchem.7b01695
89期:19页:10256-10263
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]CHEMICALLY-MODIFIED OLIGONUCLEOTIDES ; COLLISION-INDUCED DISSOCIATION ; LIQUID-CHROMATOGRAPHY ; NUCLEIC-ACIDS ; POSTTRANSLATIONAL MODIFICATIONS ; DNA OLIGONUCLEOTIDES ; PROTEOME ANALYSIS ; CHARGED DROPLETS ; CATION-EXCHANGE ; IONIZATION
英文摘要There are two challenges in oligonucleotide detection by liquid chromatography coupled with mass spectrometry (LC-MS), the serious ion suppression effects caused by ion-pair reagents and the low detection sensitivity in positive mode MS. In this study, highly concentrated alcohol vapors were introduced into an enclosed electrospray ionization chamber, and oligonucleotides could be well detected in negative mode MS even with 100 mM triethylammonium acetate (TEAA) as an ion-pair reagent. The MS signal intensity was improved 600-fold (for standard oligonucleotide dT15) by the isopropanol vapor assisted electrospray, and effective ion-pair LC separation was feasibly coupled with high-sensitive MS detection. Then, oligonucleotides were successfully detected in positive mode MS with few adducts by propanoic acid vapor assisted electrospray. The signal intensity was enhanced more than 10-fold on average compared with adding acids into the electrospray solution. Finally, oligonucleotides and peptides or histones were simultaneously detected in MS with little interference with each other. Our strategy provides a useful alternative for investigating the biological functions of oligonucleotides.
语种英语
WOS记录号WOS:000412716800023
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/150127
专题中国科学院大连化学物理研究所
作者单位1.Zhejiang Univ, Dept Chem, Hangzhou 310027, Zhejiang, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Weng, Guofeng,Liu, Zheyi,Chen, Jin,et al. Enhancing the Mass Spectrometry Sensitivity for Oligonucleotide Detection by Organic Vapor Assisted Electrospray[J]. ANALYTICAL CHEMISTRY,2017,89(19):10256-10263.
APA Weng, Guofeng,Liu, Zheyi,Chen, Jin,Wang, Fangjun,Pan, Yuanjiang,&Zhang, Yukui.(2017).Enhancing the Mass Spectrometry Sensitivity for Oligonucleotide Detection by Organic Vapor Assisted Electrospray.ANALYTICAL CHEMISTRY,89(19),10256-10263.
MLA Weng, Guofeng,et al."Enhancing the Mass Spectrometry Sensitivity for Oligonucleotide Detection by Organic Vapor Assisted Electrospray".ANALYTICAL CHEMISTRY 89.19(2017):10256-10263.
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