中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: A hollow fiber system for simple generation of human brain organoids
作者: Zhu, Yujuan1, 2, 3;  Wang, Li1, 3;  Yin, Fangchao1, 2, 3;  Yu, Yue1, 2, 3;  Wang, Yaqing1, 2, 3;  Liu, Hui1, 3;  Wang, Hui1, 2, 3;  Sun, Ning4;  Liu, Haitao1, 2, 3;  Qin, Jianhua1, 2, 3, 5
刊名: INTEGRATIVE BIOLOGY
发表日期: 2017-09-01
DOI: 10.1039/c7ib00080d
卷: 9, 期:9, 页:774-781
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
英文摘要: 3D organoids exhibit near-physiological morphogenesis and histology relying on the self-organization of human pluripotent stem cells (hPSCs), representing a new class of in vitro model for studying developmental biology and diseases. An engineered approach is highly desirable to generate sufficient organoids in a simple and efficient manner. Herein, we present a new strategy for the simple formation of massive human brain organoids from hiPSCs within a hollow fiber reactor system by combining fiber materials with the developmental biology principle. A thin and finely adjustable calcium alginate (CaA) core-shell fiber was constructed using a multilayer coaxial laminar flow microfluidic system. The meter-long hollow fibers enabled neural differentiation of hiPSCs and simple formation of abundant brain organoids in a 3D matrix. The generated brain organoids displayed essential features of human brain organogenesis, including polarized neuroepithelium, cell type heterogeneity and discrete brain regions, resembling the early brain development. This approach is simple and easy to operate, which allows for simplified formation of massive brain organoids, overcoming the tedious procedures in conventional methods. In particular, the facile and scalable characteristics of hollow fibers are compatible with real-time observation and monitoring, as well as flexible tissue manipulations for downstream biological analysis. It might also provide a new platform to advance stem cell-derived organoid models and their utility in biomedical applications.
关键词[WOS]: CEREBRAL ORGANOIDS ;  STEM-CELLS ;  MICROFIBERS ;  DISEASE ;  DYNAMICS ;  MODEL ;  CHIP
语种: 英语
WOS记录号: WOS:000411014600005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150137
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Beijing, Peoples R China
4.Fudan Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Shanghai, Peoples R China
5.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai, Peoples R China

Recommended Citation:
Zhu, Yujuan,Wang, Li,Yin, Fangchao,et al. A hollow fiber system for simple generation of human brain organoids[J]. INTEGRATIVE BIOLOGY,2017,9(9):774-781.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zhu, Yujuan]'s Articles
 [Wang, Li]'s Articles
 [Yin, Fangchao]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zhu, Yujuan]‘s Articles
 [Wang, Li]‘s Articles
 [Yin, Fangchao]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace