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Metabolic profiling analysis of Siraitia grosvenorii revealed different characteristics of green fruit and saccharified yellow fruit
Fang, Chengnan1,2; Wang, Qingqing1,2; Liu, Xinyu1,2; Xu, Guowang1,2
关键词Siraitia Grosvenorii Uplc-q-tof-ms Metabolic Profiling Saccharification Distribution Characteristics
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2017-10-25
DOI10.1016/j.jpba.2017.06.046
145页:158-168
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]FLIGHT MASS-SPECTROMETRY ; LUO-HAN-GUO ; LIQUID-CHROMATOGRAPHY ; COMPREHENSIVE ANALYSIS ; CHEMICAL-CONSTITUENTS ; MOGROSIDE V ; IN-VITRO ; GLYCOSIDES ; TRITERPENE ; IDENTIFICATION
英文摘要Siraitia grosvenorii is an economic and medicinal plant, its fruit is considered to be good to health for its diverse bioactive ingredients. However, the clarification of chemical composition and their changes after saccharification procedure are not well performed. In present study, a metabolomics method based on ultra-high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry was developed for metabolic profiling acquisition ofSiraitia grosvenorii extract. Furthermore, information dependent analysis (IDA) combined with self-constructed LC MS/MS identification system for metabolites were employed to identify primary and secondary metabolites in Siraitia grosvenorii. A total of 126 metabolites were identified or tentatively identified. The obvious differences of metabolic profiling between green fruit and saccharified yellow fruit were observed, and metabolites showed their own distribution characteristics in peel, flesh and seed. The majority of the nutrients and effective components were more distributed in flesh and peel, and saccharification was conducive to accumulation of sweet glycosides. This study not only expanded metabolite composition information of Siraitia grosvenorii, but also specified distribution characteristics of identified metabolites. (C) 2017 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000410872200021
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/150139
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Fang, Chengnan,Wang, Qingqing,Liu, Xinyu,et al. Metabolic profiling analysis of Siraitia grosvenorii revealed different characteristics of green fruit and saccharified yellow fruit[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2017,145:158-168.
APA Fang, Chengnan,Wang, Qingqing,Liu, Xinyu,&Xu, Guowang.(2017).Metabolic profiling analysis of Siraitia grosvenorii revealed different characteristics of green fruit and saccharified yellow fruit.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,145,158-168.
MLA Fang, Chengnan,et al."Metabolic profiling analysis of Siraitia grosvenorii revealed different characteristics of green fruit and saccharified yellow fruit".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 145(2017):158-168.
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