DICP OpenIR
Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS
Wang, Qi1,2; Wang, Keyun1,2; Ye, Mingliang1,2
Source PublicationANALYST
2017-10-17
DOI10.1039/c7an00954b
Volume142Issue:19Pages:3536-3548
Indexed BySCI
SubtypeReview
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordADENOSYL-L-METHIONINE ; AFFINITY-CHROMATOGRAPHY ADSORBENT ; POSITIVE-ION MODE ; MASS-SPECTROMETRY ; LYSINE-METHYLATION ; ARGININE METHYLATION ; PROTEOMIC ANALYSIS ; POSTTRANSLATIONAL MODIFICATIONS ; HUMAN-CELLS ; IN-VIVO
AbstractProtein methylation is an important post-translational modification (PTM) that plays crucial roles in the regulation of diverse biological processes. Though many efforts have been devoted to the investigation of protein methylation, the analysis of non-histone methylation at the proteome level is still a great challenge. The alteration of the protein/peptide physicochemical properties caused by methylation is very small, thus it is difficult to develop highly efficient enrichment approaches to separate methylated peptides from a pool of diverse background peptides. The mass shifts caused by methylations are identical to the substitutions of some amino acids, thus it is difficult to confidently identify methylated peptides. In this review, we report on recent advances in the development of methods for large-scale analysis of nonhistone protein methylation. Especially the methods for efficient enrichment and the approaches for controlling identification confidence have been covered.
Language英语
WOS IDWOS:000411703800001
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150148
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Qi,Wang, Keyun,Ye, Mingliang. Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS[J]. ANALYST,2017,142(19):3536-3548.
APA Wang, Qi,Wang, Keyun,&Ye, Mingliang.(2017).Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS.ANALYST,142(19),3536-3548.
MLA Wang, Qi,et al."Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS".ANALYST 142.19(2017):3536-3548.
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