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题名: Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis
作者: Liu, Lukuan1, 2, 3;  Yang, Kaiguang1;  Zhu, Xudong1, 2;  Liang, Yu1;  Chen, Yuanbo1, 2;  Fang, Fei1, 2;  Zhao, Qun1;  Zhang, Lihua1;  Zhang, Yukui1
关键词: Circulating tumor cells ;  Aptamer ;  Proteome ;  Open tubular column
刊名: TALANTA
发表日期: 2017-12-01
DOI: 10.1016/j.talanta.2017.07.041
卷: 175, 页:189-193
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Circulating tumor cells hold the key to predicting the prognosis and discovering the therapeutic targets. Herein, we proposed a strategy to develop an aptamer-immobilized open tubular capillary column by which SMMC-7721 human hepatoma cells (SMMC-7721 cells) could be captured with an over 70% of capture efficiency and a 3.0 +/- 0.2 of enrichment factor. Owing to the compatibility of the column, the captured cells by the column could be analyzed by LC-MS from protein level and 5 unique proteins of SMMC-7721 cells were identified which could be used as markers to identify SMMC-7721 cells when Jurkat T-leukemia cells (Jurkat cells) were employed as interfering cells. As the key component, the aptamer-immobilized column had the potential to be integrated into the platform for separating, enriching and characterizing rare cells simultaneously.
关键词[WOS]: MULTIPLE TYPES ;  NANOPARTICLES ;  ENRICHMENT ;  CANCER ;  ATTACHMENT ;  CHALLENGES ;  SEPARATION ;  SURFACES ;  POLYMER
语种: 英语
WOS记录号: WOS:000412033100024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150163
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China

Recommended Citation:
Liu, Lukuan,Yang, Kaiguang,Zhu, Xudong,et al. Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis[J]. TALANTA,2017,175:189-193.
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