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High-throughput Screening of Potential Allergens from Complex Proteins of Large Yellow Croaker (Larimichthys crocea) by Mass Spectrometry
Yu Yang1,2; Gao Qing-Li1; Song Yang1; Zhang Jing-Yu1; Li Zhi-Bo1; Li Wei1; Jin Yan2; Li Meng1; Zhao Qian-Cheng1; Qi Yan-Xia1
KeywordLarimichthys Crocea Allergens Mass Spectrometry Sequence Homology Analysis Simulated Gastric Fluid Digestion
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2017-05-01
DOI10.1016/S1872-2040(17)61016-8
Volume45Issue:5Pages:1713-1718
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordFOOD ALLERGENS ; LIQUID-CHROMATOGRAPHY ; FRAGRANCE ALLERGENS ; IN-VITRO ; DIGESTION ; FISH ; PARVALBUMIN ; STABILITY ; SHOTGUN ; WATER
AbstractThe allergenicity of large yellow croaker (Larimichthys crocea) proteins has been widely reported to cause many serious food safety problems. In this study, the protein sample extracted from Larimichthys crocea was digested and analyzed by reversed phase liquid chromatography coupled with tandem mass spectrometry (RPLC-MS/MS) for globally proteomic analysis, and the Larimichthys crocea proteins were digested with simulated gastric fluid (SGF) at different time intervals and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The digestion-resistant proteins were further done with in-gel digestion and analyzed by RPLC-MS/MS. Then, sequence homology analysis between identified proteins and known allergens was performed. Results showed that 33 kinds of distinct proteins were feasibly identified from Larimichthys crocea by RPLC-MS/MS and the protein band (approximately 44.0 kDa) presented in SDS-PAGE was considered as protein-resistant to SGF digestion. Two potential allergens, beta-enolase and tropomyosin alpha-1 chain, were found from proteins resistant to SGF digestion by sequence homology analysis. It was observed that most of these SGF digestion-resistant proteins were potential allergens with high sequences similarity to the known protein allergens from other species. Therefore, this strategy provides an efficient alternative for potential allergens screening with high-throughput, which exhibits great potential application in fish protein allergens related food safety control.
Language英语
WOS IDWOS:000412224800001
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150183
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Ocean Univ, Coll Food Sci & Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Yu Yang,Gao Qing-Li,Song Yang,et al. High-throughput Screening of Potential Allergens from Complex Proteins of Large Yellow Croaker (Larimichthys crocea) by Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2017,45(5):1713-1718.
APA Yu Yang.,Gao Qing-Li.,Song Yang.,Zhang Jing-Yu.,Li Zhi-Bo.,...&Qi Yan-Xia.(2017).High-throughput Screening of Potential Allergens from Complex Proteins of Large Yellow Croaker (Larimichthys crocea) by Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,45(5),1713-1718.
MLA Yu Yang,et al."High-throughput Screening of Potential Allergens from Complex Proteins of Large Yellow Croaker (Larimichthys crocea) by Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 45.5(2017):1713-1718.
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