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题名: MicroRNA-205-5p regulates the chemotherapeutic resistance of hepatocellular carcinoma cells by targeting PTEN/JNK/ANXA3 pathway
作者: Shao, Ping1;  Qu, Wei-Kun1;  Wang, Cheng-Ye1;  Tian, Yu1;  Ye, Ming-Liang2;  Sun, De-Guang1;  Sui, Ji-Dong1;  Wang, Li-Ming1;  Fan, Rong3;  Gao, Zhen-Ming1
关键词: MicroRNA-205 ;  hepatocellular carcinoma ;  chemotherapeutic resistance ;  PTEN
刊名: AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
发表日期: 2017
卷: 9, 期:9, 页:4300-+
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Oncology ;  Medicine, Research & Experimental
研究领域[WOS]: Oncology ;  Research & Experimental Medicine
英文摘要: Hepatocellular carcinoma (HCC) is a common malignant tumor of the digestive system, and patients with advanced HCC have a poor outlook, partly due to resistance to chemotherapeutic drugs. Previous studies have implicated microRNAs in the regulation of chemoresistance, and we have previously shown that microRNA (miR)-205- 5p is down-regulated in multiple hepatoma cell lines. Here, we investigate whether miR-205-5p is involved in chemotherapeutic resistance in HCC. Expression of miR-205-5p was measured by real-time quantitative reverse transcription PCR and cell viability was determined using a CCK-8 cell viability assay. Expression of proteins in the PTEN/JNK/ANXA3 pathway were assessed via Western blotting. We found that miR-205-5p expression was down-regulated in all HCC cell lines investigated. In addition, miR-205-5p expression was upregulated by 5-fluorouracil (5-Fu) treatment in Bel-7402 (Bel) cells. Interestingly, miR-205-5p expression was increased in multidrug-resistant Bel-7402/5-Fu (Bel/Fu) cells, compared with Bel cells. We next demonstrated that sensitivity to 5-Fu was increased in Bel/Fu cells after treatment with a miR-205-5p inhibitor. Similarly, increased resistance to 5-Fu was observed in Bel cells after transfection with a miR-205-5p mimic. We injected nude mice with Bel/5-Fu cells to promote tumor growth, and found that co-treatment with a miR-205-5p antagomir and 5-Fu slowed tumor growth more than either treatment alone. Finally, we found that these effects were all associated with changes in the PTEN/JNK/ANXA3 pathway. In conclusion, inhibition of miR-205-5p may reverse chemotherapeutic resistance to 5-Fu, and this may occur via the PTEN/JNK/ANXA3 pathway.
关键词[WOS]: COLORECTAL-CANCER CELLS ;  TUMOR-SUPPRESSOR ;  MIR-205 ;  PTEN ;  PROLIFERATION ;  EXPRESSION ;  PROMOTES ;  PROTEIN ;  GROWTH ;  CHEMORESISTANCE
语种: 英语
WOS记录号: WOS:000412145400038
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150204
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, Affiliated Hosp 2, Dept Surg, Div Hepatobiliary & Pancreat Surg, 467 Zhongshan Rd, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, VIP Ward 2, 467 Zhongshan Rd, Dalian 116023, Peoples R China

Recommended Citation:
Shao, Ping,Qu, Wei-Kun,Wang, Cheng-Ye,et al. MicroRNA-205-5p regulates the chemotherapeutic resistance of hepatocellular carcinoma cells by targeting PTEN/JNK/ANXA3 pathway[J]. AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,2017,9(9):4300-+.
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