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A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate
Qiao, Qinglong1,2; Liu, Wenjuan2; Chen, Jie2; Zhou, Wei1,2; Yin, Wenting2; Miao, Lu2; Cui, Jingnan1; Xu, Zhaochao2
KeywordFluorogenic Probe Snap-tag Labeling Rate Protein Imaging Wash Free
Source PublicationDYES AND PIGMENTS
2017-12-01
DOI10.1016/j.dyepig.2017.08.032
Volume147Pages:327-333
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
WOS SubjectChemistry, Applied ; Engineering, Chemical ; Materials Science, Textiles
WOS Research AreaChemistry ; Engineering ; Materials Science
WOS KeywordLIVING CELLS ; FLUORESCENT-PROBE ; PROTEIN ; FLUOROPHORE
AbstractSNAP-tag is one of most popular genetically encoded protein tags that can be labeled with fluorescent molecules for visualizing a protein of interest in live cells. Fluorogenic probes keep dark until they label protein tags, significantly improving the signal-to-noise ratio to image proteins without wash-out step. However, most of reported fluorogenic probes for SNAP-tag suffered from the low or mild labeling rate comparing with non-fluorogenic ones. In this paper, we reported a 4-amino-naphthliamide derived fluorogenic probe for SNAP-tag, which exhibited the fast record labeling rate among fluorogenic probes. Finally, we applied this probe to image proteins in mitochondria and nucleus in live cells without washout steps. (C) 2017 Elsevier Ltd. All rights reserved.
Language英语
WOS IDWOS:000412612400037
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150205
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Qiao, Qinglong,Liu, Wenjuan,Chen, Jie,et al. A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate[J]. DYES AND PIGMENTS,2017,147:327-333.
APA Qiao, Qinglong.,Liu, Wenjuan.,Chen, Jie.,Zhou, Wei.,Yin, Wenting.,...&Xu, Zhaochao.(2017).A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate.DYES AND PIGMENTS,147,327-333.
MLA Qiao, Qinglong,et al."A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate".DYES AND PIGMENTS 147(2017):327-333.
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