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题名: Selective separation of xanthones and saponins from the rhizomes of Anemarrhena asphodeloides by modulating the density of surface charges in C18-bonded stationary phases
作者: Cai, Jianfeng1;  Xin, Huaxia1;  Cheng, Lingping1;  Guo, Zhimou2;  Feng, Jiatao2;  Fu, Qing1;  Jin, Yu1;  Liang, Xinmiao1, 2
刊名: ANALYTICAL METHODS
发表日期: 2017-10-14
DOI: 10.1039/c7ay01667k
卷: 9, 期:38, 页:5604-5610
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Chemistry, Analytical ;  Food Science & Technology ;  Spectroscopy
研究领域[WOS]: Chemistry ;  Food Science & Technology ;  Spectroscopy
英文摘要: A rapid and robust separation method based on the positively charged reversed-phase (PGRP) stationary phase was developed for selective separation of saponins and xanthones from the rhizomes of Anemarrhena asphodeloides (A. asphodeloides). In this work, the chromatographic performances of three PGRP stationary phases with different surface positive charge densities were systematically evaluated by studying hydrophobicity, effects of pH and buffer concentration of the mobile phase, ion-exchange capacity, etc. The PGRP stationary phase exhibited reversed-phase/anion-exchange mixed-mode properties. Then the retention behaviors of xanthones were investigated. A good retention of xanthones was obtained at high pH and xanthones were easily eluted at low pH. The pH is like an on-off switch on the PGRP stationary phase that controls the retention of xanthones. Finally, this PGRP material was successfully applied to the selective separation of saponins and xanthones from A. asphodeloides. The result demonstrated that neutral and ionic compounds, such as xanthones and saponins from the rhizomes of A. asphodeloides, were separated selectively by modulating both the density of surface charges in the PGRP stationary phase and the pH of the mobile phase.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  IONIZABLE COMPOUNDS ;  STEROIDAL SAPONINS ;  ADSORPTION PROCESS ;  MASS-SPECTROMETRY ;  IONIC-STRENGTH ;  PH ;  PURIFICATION
语种: 英语
WOS记录号: WOS:000412945300006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150222
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.East China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116203, Peoples R China

Recommended Citation:
Cai, Jianfeng,Xin, Huaxia,Cheng, Lingping,et al. Selective separation of xanthones and saponins from the rhizomes of Anemarrhena asphodeloides by modulating the density of surface charges in C18-bonded stationary phases[J]. ANALYTICAL METHODS,2017,9(38):5604-5610.
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