DICP OpenIR
Microchip-based single-cell functional proteomics for biomedical applications
Lu, Yao1; Yang, Liu2; Wei, Wei3,4; Shi, Qihui2
Source PublicationLAB ON A CHIP
2017-04-07
DOI10.1039/c7lc00037e
Volume17Issue:7Pages:1250-1263
Indexed BySCI
SubtypeReview
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS KeywordDROPLET-BASED MICROFLUIDICS ; MASS-SPECTROMETRY ; CANCER-CELLS ; FLOW-CYTOMETRY ; T-CELLS ; CYTOKINE SECRETION ; PROTEINS ; HETEROGENEITY ; DRUG ; EXPRESSION
AbstractCellular heterogeneity has been widely recognized but only recently have single cell tools become available that allow characterizing heterogeneity at the genomic and proteomic levels. We review the technological advances in microchip-based toolkits for single-cell functional proteomics. Each of these tools has distinct advantages and limitations, and a few have advanced toward being applied to address biological or clinical problems that traditional population-based methods fail to address. High-throughput single-cell proteomic assays generate high-dimensional data sets that contain new information and thus require developing new analytical frameworks to extract new biology. In this review article, we highlight a few biological and clinical applications in which microchip-based single-cell proteomic tools provide unique advantages. The examples include resolving functional heterogeneity and dynamics of immune cells, dissecting cell-cell interaction by creating a well-controlled on-chip microenvironment, capturing high-resolution snapshots of immune system functions in patients for better immunotherapy and elucidating phosphoprotein signaling networks in cancer cells for guiding effective molecularly targeted therapies.
Language英语
WOS IDWOS:000398955800004
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150334
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
2.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Minist Educ, Key Lab Syst Biomed, Shanghai, Peoples R China
3.Univ Calif Los Angeles, David Geffen Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA
4.Univ Calif Los Angeles, David Geffen Sch Med, Jonsson Comprehens Canc Ctr, Los Angeles, CA 90095 USA
Recommended Citation
GB/T 7714
Lu, Yao,Yang, Liu,Wei, Wei,et al. Microchip-based single-cell functional proteomics for biomedical applications[J]. LAB ON A CHIP,2017,17(7):1250-1263.
APA Lu, Yao,Yang, Liu,Wei, Wei,&Shi, Qihui.(2017).Microchip-based single-cell functional proteomics for biomedical applications.LAB ON A CHIP,17(7),1250-1263.
MLA Lu, Yao,et al."Microchip-based single-cell functional proteomics for biomedical applications".LAB ON A CHIP 17.7(2017):1250-1263.
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