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题名: Microchip-based single-cell functional proteomics for biomedical applications
作者: Lu, Yao1;  Yang, Liu2;  Wei, Wei3, 4;  Shi, Qihui2
刊名: LAB ON A CHIP
发表日期: 2017-04-07
DOI: 10.1039/c7lc00037e
卷: 17, 期:7, 页:1250-1263
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Multidisciplinary ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Science & Technology - Other Topics
英文摘要: Cellular heterogeneity has been widely recognized but only recently have single cell tools become available that allow characterizing heterogeneity at the genomic and proteomic levels. We review the technological advances in microchip-based toolkits for single-cell functional proteomics. Each of these tools has distinct advantages and limitations, and a few have advanced toward being applied to address biological or clinical problems that traditional population-based methods fail to address. High-throughput single-cell proteomic assays generate high-dimensional data sets that contain new information and thus require developing new analytical frameworks to extract new biology. In this review article, we highlight a few biological and clinical applications in which microchip-based single-cell proteomic tools provide unique advantages. The examples include resolving functional heterogeneity and dynamics of immune cells, dissecting cell-cell interaction by creating a well-controlled on-chip microenvironment, capturing high-resolution snapshots of immune system functions in patients for better immunotherapy and elucidating phosphoprotein signaling networks in cancer cells for guiding effective molecularly targeted therapies.
关键词[WOS]: DROPLET-BASED MICROFLUIDICS ;  MASS-SPECTROMETRY ;  CANCER-CELLS ;  FLOW-CYTOMETRY ;  T-CELLS ;  CYTOKINE SECRETION ;  PROTEINS ;  HETEROGENEITY ;  DRUG ;  EXPRESSION
语种: 英语
WOS记录号: WOS:000398955800004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150334
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
2.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Minist Educ, Key Lab Syst Biomed, Shanghai, Peoples R China
3.Univ Calif Los Angeles, David Geffen Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA
4.Univ Calif Los Angeles, David Geffen Sch Med, Jonsson Comprehens Canc Ctr, Los Angeles, CA 90095 USA

Recommended Citation:
Lu, Yao,Yang, Liu,Wei, Wei,et al. Microchip-based single-cell functional proteomics for biomedical applications[J]. LAB ON A CHIP,2017,17(7):1250-1263.
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