中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples
作者: Wang, Yi-Ru1, 2;  Feng, Lei1, 3;  Xu, Liang4;  Hou, Jie1, 3;  Jin, Qiang2;  Zhou, Nan1;  Lin, Yuan1;  Cui, Jing-Nan3;  Ge, Guang-Bo2, 3
关键词: Human albumin (HA) ;  Fluorescent probe ;  Ultrahigh sensitivity ;  Conformation sensitive ;  Ligand-binding sites
刊名: SENSORS AND ACTUATORS B-CHEMICAL
发表日期: 2017-06-01
DOI: 10.1016/j.snb.2017.02.046
卷: 245, 页:923-931
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Analytical ;  Electrochemistry ;  Instruments & Instrumentation
研究领域[WOS]: Chemistry ;  Electrochemistry ;  Instruments & Instrumentation
英文摘要: In this study, an ultrasensitive and conformation sensitive fluorescent probe HMM (2-((6hydroxynaphthalen-2-yl) methylene) malononitrile) was developed for highly selective detection of human albumin (HA) in complex biological samples. Under physiological conditions, HMM could selectively bind on HA and form a complex which exhibited strong fluorescence signal around 562 nm. Both Job's Plot analysis and displacement assays using a series of site markers of HA demonstrated that HMM could simultaneously bind on two major ligand-binding sites (site I and site II) of HA, which partially explained why HMM could trigger more than 300-fold enhancement in fluorescence signal. The newly developed probe exhibited ultrahigh sensitivity (LOD =50 ngmL(-1)) and excellent selectivity toward HA over other human serum proteins. HMM has been successfully used to detect HA in diluted human plasma and urine, and the results demonstrate that such detection is highly reliable. Unlike bromocresol green (a commonly used dye-binding probe of HA), HMM is a conformation sensitive fluorescent probe for HA and it could distinguish between native and denatured HA. All these features demonstrated that HMM could serve as a practical and powerful tool for HA associated clinical routine tests including quantification of serum and urinary albumin. (C) 2017 Elsevier B.V. All rights reserved.
关键词[WOS]: HUMAN SERUM-ALBUMIN ;  HUMAN CARBOXYLESTERASE 2 ;  EMISSION CHARACTERISTICS ;  SELECTIVE DETECTION ;  BINDING ;  PLASMA ;  DRUG ;  PROTEINS ;  IDENTIFICATION ;  NANOPARTICLES
语种: 英语
WOS记录号: WOS:000398760700108
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150335
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Dalian Med Univ, Dalian 116044, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian, Peoples R China
4.Dalian Univ Technol, Sch Chem, Dalian, Peoples R China

Recommended Citation:
Wang, Yi-Ru,Feng, Lei,Xu, Liang,et al. An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples[J]. SENSORS AND ACTUATORS B-CHEMICAL,2017,245:923-931.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Wang, Yi-Ru]'s Articles
 [Feng, Lei]'s Articles
 [Xu, Liang]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Wang, Yi-Ru]‘s Articles
 [Feng, Lei]‘s Articles
 [Xu, Liang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace