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An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples
Wang, Yi-Ru1,2; Feng, Lei1,3; Xu, Liang4; Hou, Jie1,3; Jin, Qiang2; Zhou, Nan1; Lin, Yuan1; Cui, Jing-Nan3; Ge, Guang-Bo2,3
KeywordHuman Albumin (Ha) Fluorescent Probe Ultrahigh Sensitivity Conformation Sensitive Ligand-binding Sites
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
2017-06-01
DOI10.1016/j.snb.2017.02.046
Volume245Pages:923-931
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS KeywordHUMAN SERUM-ALBUMIN ; HUMAN CARBOXYLESTERASE 2 ; EMISSION CHARACTERISTICS ; SELECTIVE DETECTION ; BINDING ; PLASMA ; DRUG ; PROTEINS ; IDENTIFICATION ; NANOPARTICLES
AbstractIn this study, an ultrasensitive and conformation sensitive fluorescent probe HMM (2-((6hydroxynaphthalen-2-yl) methylene) malononitrile) was developed for highly selective detection of human albumin (HA) in complex biological samples. Under physiological conditions, HMM could selectively bind on HA and form a complex which exhibited strong fluorescence signal around 562 nm. Both Job's Plot analysis and displacement assays using a series of site markers of HA demonstrated that HMM could simultaneously bind on two major ligand-binding sites (site I and site II) of HA, which partially explained why HMM could trigger more than 300-fold enhancement in fluorescence signal. The newly developed probe exhibited ultrahigh sensitivity (LOD =50 ngmL(-1)) and excellent selectivity toward HA over other human serum proteins. HMM has been successfully used to detect HA in diluted human plasma and urine, and the results demonstrate that such detection is highly reliable. Unlike bromocresol green (a commonly used dye-binding probe of HA), HMM is a conformation sensitive fluorescent probe for HA and it could distinguish between native and denatured HA. All these features demonstrated that HMM could serve as a practical and powerful tool for HA associated clinical routine tests including quantification of serum and urinary albumin. (C) 2017 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000398760700108
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150335
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Med Univ, Dalian 116044, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian, Peoples R China
4.Dalian Univ Technol, Sch Chem, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yi-Ru,Feng, Lei,Xu, Liang,et al. An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples[J]. SENSORS AND ACTUATORS B-CHEMICAL,2017,245:923-931.
APA Wang, Yi-Ru.,Feng, Lei.,Xu, Liang.,Hou, Jie.,Jin, Qiang.,...&Ge, Guang-Bo.(2017).An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples.SENSORS AND ACTUATORS B-CHEMICAL,245,923-931.
MLA Wang, Yi-Ru,et al."An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples".SENSORS AND ACTUATORS B-CHEMICAL 245(2017):923-931.
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