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An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples
Wang, Yi-Ru1,2; Feng, Lei1,3; Xu, Liang4; Hou, Jie1,3; Jin, Qiang2; Zhou, Nan1; Lin, Yuan1; Cui, Jing-Nan3; Ge, Guang-Bo2,3
关键词Human Albumin (Ha) Fluorescent Probe Ultrahigh Sensitivity Conformation Sensitive Ligand-binding Sites
刊名SENSORS AND ACTUATORS B-CHEMICAL
2017-06-01
DOI10.1016/j.snb.2017.02.046
245页:923-931
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
研究领域[WOS]Chemistry ; Electrochemistry ; Instruments & Instrumentation
关键词[WOS]HUMAN SERUM-ALBUMIN ; HUMAN CARBOXYLESTERASE 2 ; EMISSION CHARACTERISTICS ; SELECTIVE DETECTION ; BINDING ; PLASMA ; DRUG ; PROTEINS ; IDENTIFICATION ; NANOPARTICLES
英文摘要In this study, an ultrasensitive and conformation sensitive fluorescent probe HMM (2-((6hydroxynaphthalen-2-yl) methylene) malononitrile) was developed for highly selective detection of human albumin (HA) in complex biological samples. Under physiological conditions, HMM could selectively bind on HA and form a complex which exhibited strong fluorescence signal around 562 nm. Both Job's Plot analysis and displacement assays using a series of site markers of HA demonstrated that HMM could simultaneously bind on two major ligand-binding sites (site I and site II) of HA, which partially explained why HMM could trigger more than 300-fold enhancement in fluorescence signal. The newly developed probe exhibited ultrahigh sensitivity (LOD =50 ngmL(-1)) and excellent selectivity toward HA over other human serum proteins. HMM has been successfully used to detect HA in diluted human plasma and urine, and the results demonstrate that such detection is highly reliable. Unlike bromocresol green (a commonly used dye-binding probe of HA), HMM is a conformation sensitive fluorescent probe for HA and it could distinguish between native and denatured HA. All these features demonstrated that HMM could serve as a practical and powerful tool for HA associated clinical routine tests including quantification of serum and urinary albumin. (C) 2017 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000398760700108
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/150335
专题中国科学院大连化学物理研究所
作者单位1.Dalian Med Univ, Dalian 116044, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian, Peoples R China
4.Dalian Univ Technol, Sch Chem, Dalian, Peoples R China
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Wang, Yi-Ru,Feng, Lei,Xu, Liang,et al. An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples[J]. SENSORS AND ACTUATORS B-CHEMICAL,2017,245:923-931.
APA Wang, Yi-Ru.,Feng, Lei.,Xu, Liang.,Hou, Jie.,Jin, Qiang.,...&Ge, Guang-Bo.(2017).An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples.SENSORS AND ACTUATORS B-CHEMICAL,245,923-931.
MLA Wang, Yi-Ru,et al."An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples".SENSORS AND ACTUATORS B-CHEMICAL 245(2017):923-931.
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