中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Identification and characterization of naturally occurring inhibitors against human carboxylesterase 2 in White Mulberry Root-bark
作者: Liu, Ya-Jing1;  Li, Shi-Yang2;  Hou, Jie1, 3;  Liu, Yan-Fang2;  Wang, Dan-Dan2;  Jiang, Yong-Shan1, 4;  Ge, Guang-Bo2, 3;  Liang, Xin-Miao2;  Yang, Ling2
关键词: White Mulberry Root-bark ;  Human carboxylesterase 2 (hCE2) ;  Inhibitory effects ;  Sanggenone D ;  Kuwanon G ;  Sanggenone C
刊名: FITOTERAPIA
发表日期: 2016-12-01
DOI: 10.1016/j.fitote.2016.09.022
卷: 115, 页:57-63
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Medicinal ;  Pharmacology & Pharmacy
英文摘要: White Mulberry Root-bark (WMR) is an edible Chinese herbal used for the treatment of inflammation, nephritis and asthma. This study aimed to investigate the inhibitory effects of ethanol extract from WMR against human carboxylesterase 2 (hCE2), as well as to identity and character natural hCE2 inhibitors in this herbal. Our results demonstrated that the ethanol extract of WMR displayed potent inhibitory effects against hCE2, while three major bioactive constitutes in WMR were identified on the basis of LC fingerprinting combined with activity based screening of LC fractions. Three bioactive compounds including SD, KG and SC were efficiently identified by comparison of LC retention times, UV and MS spectral data, with the help of authentic standards. The inhibition potentials and inhibition types of these natural compounds against hCE2 were further investigated in human liver microsomes. The results demonstrated that these bioactive compounds are potent non-competitive inhibitors against hCE2, with the K-i values ranging from 0.76 mu M to 1.09 mu M. All these findings suggested that three abundant natural compounds in WMR displayed potent inhibitory effects against hCE2, which could be used as lead compounds to develop more potent hCE2 inhibitors for the alleviation of hCE2-mediated severe delayed onset diarrhea. (C) 2016 Published by Elsevier B.V.
关键词[WOS]: HUMAN UDP-GLUCURONOSYLTRANSFERASES ;  MORUS-ALBA ;  SELECTIVE INHIBITORS ;  BIOLOGICAL-ACTIVITY ;  IRINOTECAN CPT-11 ;  COLORECTAL-CANCER ;  FLUORESCENT-PROBE ;  NITRIC-OXIDE ;  METABOLISM ;  POTENT
语种: 英语
WOS记录号: WOS:000389113300010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150351
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Dalian Med Univ, Dalian 116044, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
4.Dalian Med Univ, Affiliated Hosp 1, Dalian 116011, Peoples R China

Recommended Citation:
Liu, Ya-Jing,Li, Shi-Yang,Hou, Jie,et al. Identification and characterization of naturally occurring inhibitors against human carboxylesterase 2 in White Mulberry Root-bark[J]. FITOTERAPIA,2016,115:57-63.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Liu, Ya-Jing]'s Articles
 [Li, Shi-Yang]'s Articles
 [Hou, Jie]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Liu, Ya-Jing]‘s Articles
 [Li, Shi-Yang]‘s Articles
 [Hou, Jie]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace