DICP OpenIR
Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses
Li, Lili1,2; Zhao, Jieyu1,2; Zhao, Yanni1,2; Lu, Xin1,2; Zhou, Zhihui1,2; Zhao, Chunxia1,2; Xu, Guowang1,2
Source PublicationSCIENTIFIC REPORTS
2016-11-29
DOI10.1038/srep37976
Volume6
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS KeywordLEAF SENESCENCE ; NICOTIANA-TABACUM ; MASS-SPECTROMETRY ; TRANSGENIC TOBACCO ; ARABIDOPSIS ; PLANTS ; STRESS ; BIOSYNTHESIS ; METABOLISM ; STEROLS
AbstractSenescence is the final stage of leaf growth and development. Many different physiological activities occur during this process. A comprehensive metabolomics analysis of tobacco middle leaves at 5 different developmental stages was implemented through multi-platform methods based on liquid chromatography, capillary electrophoresis and gas chromatography coupled with mass spectrometry. In total, 412 metabolites were identified, including pigments, sterols, lipids, amino acids, polyamines, sugars and secondary metabolites. Dramatic metabolic changes were observed. Firstly, membrane degradation and chlorophyll down-regulation occurred after the 50% flower bud stage. Levels of major membrane lipids decreased, including those of the glycolipids in chloroplast thylakoids and phospholipids in membrane envelopes. Clear decreases in free sterols and acylated sterol glucosides were detected along with the accumulation of sterol esters. The accumulation of alkaloids was found. The amino acid levels were significantly decreased, particularly those of N-rich amino acids (glutamine and asparagine), thus reflecting N translocation. Subsequently, the antioxidant system was activated. Sugar alcohols and polyphenols accumulated when the lower leaves turned yellow. These results comprehensively revealed the metabolic changes that occur during tobacco leaf development and senescence under natural conditions.
Language英语
WOS IDWOS:000388662600004
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150357
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Lili,Zhao, Jieyu,Zhao, Yanni,et al. Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses[J]. SCIENTIFIC REPORTS,2016,6.
APA Li, Lili.,Zhao, Jieyu.,Zhao, Yanni.,Lu, Xin.,Zhou, Zhihui.,...&Xu, Guowang.(2016).Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses.SCIENTIFIC REPORTS,6.
MLA Li, Lili,et al."Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses".SCIENTIFIC REPORTS 6(2016).
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