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Antibody-Free Approach for the Global Analysis of Protein Methylation
Wang, Keyun1,2; Dong, Mingming1,2; Mao, Jiawei1,2; Wang, Yan1,2; Jin, Yan1,2; Ye, Mingliang1,2; Zou, Hanfa1,2
刊名ANALYTICAL CHEMISTRY
2016-12-06
DOI10.1021/acs.analchem.6b02872
88期:23页:11319-11327
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
关键词[WOS]LARGE-SCALE IDENTIFICATION ; ADENOSYL-L-METHIONINE ; QUANTIFYING IN-VIVO ; ARGININE METHYLATION ; MASS-SPECTROMETRY ; LYSINE METHYLATION ; PROTEOMIC ANALYSIS ; HUMAN-CELLS ; REVEALS ; ENRICHMENT
英文摘要Protein methylation is receiving more and more attention for its important regulating role in diverse biological processes including epigenetic regulation of gene transcription, RNA processing, DNA damage repair, and signal transduction. Global analysis of protein methylation at the proteome level requires the enrichment of methylated peptides with various forms; unfortunately, the immunoaffinity purification method can only enrich a subset of them due to lacking of pan specific antibody. Because methylation does not significantly alter the physicochemical properties of arginine or lysine residues, chemical approach for global methylome analysis is still at infancy. In this study, by exploiting the fact that the methylation on Arg and Lys prohibiting the cleavage by proteases for these sites, we developed an antibody-free method to enrich methylated peptides, which enabled the identification of 887 methylation forms on 768 sites from HepG2 cells. This technique allows the simultaneous analysis of both Lys and Arg methylation while it has better performance for the identification of Arg methylation. It should find broad applications in studying methylation regulated biological processes.
语种英语
WOS记录号WOS:000389556900012
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/150414
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Wang, Keyun,Dong, Mingming,Mao, Jiawei,et al. Antibody-Free Approach for the Global Analysis of Protein Methylation[J]. ANALYTICAL CHEMISTRY,2016,88(23):11319-11327.
APA Wang, Keyun.,Dong, Mingming.,Mao, Jiawei.,Wang, Yan.,Jin, Yan.,...&Zou, Hanfa.(2016).Antibody-Free Approach for the Global Analysis of Protein Methylation.ANALYTICAL CHEMISTRY,88(23),11319-11327.
MLA Wang, Keyun,et al."Antibody-Free Approach for the Global Analysis of Protein Methylation".ANALYTICAL CHEMISTRY 88.23(2016):11319-11327.
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