DICP OpenIR
Antibody-Free Approach for the Global Analysis of Protein Methylation
Wang, Keyun1,2; Dong, Mingming1,2; Mao, Jiawei1,2; Wang, Yan1,2; Jin, Yan1,2; Ye, Mingliang1,2; Zou, Hanfa1,2
Source PublicationANALYTICAL CHEMISTRY
2016-12-06
DOI10.1021/acs.analchem.6b02872
Volume88Issue:23Pages:11319-11327
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS KeywordLARGE-SCALE IDENTIFICATION ; ADENOSYL-L-METHIONINE ; QUANTIFYING IN-VIVO ; ARGININE METHYLATION ; MASS-SPECTROMETRY ; LYSINE METHYLATION ; PROTEOMIC ANALYSIS ; HUMAN-CELLS ; REVEALS ; ENRICHMENT
AbstractProtein methylation is receiving more and more attention for its important regulating role in diverse biological processes including epigenetic regulation of gene transcription, RNA processing, DNA damage repair, and signal transduction. Global analysis of protein methylation at the proteome level requires the enrichment of methylated peptides with various forms; unfortunately, the immunoaffinity purification method can only enrich a subset of them due to lacking of pan specific antibody. Because methylation does not significantly alter the physicochemical properties of arginine or lysine residues, chemical approach for global methylome analysis is still at infancy. In this study, by exploiting the fact that the methylation on Arg and Lys prohibiting the cleavage by proteases for these sites, we developed an antibody-free method to enrich methylated peptides, which enabled the identification of 887 methylation forms on 768 sites from HepG2 cells. This technique allows the simultaneous analysis of both Lys and Arg methylation while it has better performance for the identification of Arg methylation. It should find broad applications in studying methylation regulated biological processes.
Language英语
WOS IDWOS:000389556900012
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150414
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Keyun,Dong, Mingming,Mao, Jiawei,et al. Antibody-Free Approach for the Global Analysis of Protein Methylation[J]. ANALYTICAL CHEMISTRY,2016,88(23):11319-11327.
APA Wang, Keyun.,Dong, Mingming.,Mao, Jiawei.,Wang, Yan.,Jin, Yan.,...&Zou, Hanfa.(2016).Antibody-Free Approach for the Global Analysis of Protein Methylation.ANALYTICAL CHEMISTRY,88(23),11319-11327.
MLA Wang, Keyun,et al."Antibody-Free Approach for the Global Analysis of Protein Methylation".ANALYTICAL CHEMISTRY 88.23(2016):11319-11327.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Keyun]'s Articles
[Dong, Mingming]'s Articles
[Mao, Jiawei]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Keyun]'s Articles
[Dong, Mingming]'s Articles
[Mao, Jiawei]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Keyun]'s Articles
[Dong, Mingming]'s Articles
[Mao, Jiawei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.