DICP OpenIR
Au-cysteine modified macroporous adsorption resin: preparation and highly selective enrichment and identification of N-linked glycopeptides from the complex biological sample
Sun, Xudong1; Dong, Jing2; Li, Jinan2; Ye, Mingliang2; Ou, Junjie2; Zhang, Lingyi1; Zhang, Weibing1
Source PublicationRSC ADVANCES
2016
DOI10.1039/c6ra24236g
Volume6Issue:114Pages:113058-113065
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS KeywordHYDROPHILIC INTERACTION CHROMATOGRAPHY ; FUNCTIONALIZED MAGNETIC NANOPARTICLES ; MASS-SPECTROMETRY ; EFFICIENT ENRICHMENT ; GLYCOPROTEINS ; GLYCOSYLATION ; SEPARATION ; HETEROGENEITY ; PURIFICATION ; REBAUDIOSIDE
AbstractThe development of hydrophilic stationary phase for highly efficient and selective enrichment and identification of low-abundance glycopeptides from a complex biological sample is an important prerequisite in glycoproteomics research. Herein, a hydrophilic cysteine functionalized macroporous adsorption resin/gold nanoparticle (MAR/Au-Cys) was synthesized and applied to the highly selective enrichment and identification of N-linked glycopeptides. The unique porous structure and high specific surface area of MAR provide abundant bonding sites for the immobilization of Au nanoparticles, and subsequently for the loading of cysteine via robust Au-S bonds. The structure and chemical composition of MAR/Au-Cys were confirmed by scanning electron microscopy, nitrogen adsorption-desorption analysis, mercury intrusion porosimetry and Fourier-transform infrared spectroscopy. Besides, the MAR/Au-Cys exhibited good performance in glycopeptide enrichment of tryptic digests from human immunoglobulin G and horseradish peroxidase by operation of a HILIC-SPE tip, revealing good universality, high enrichment selectivity, low detection limit (2.5 fmol) and satisfactory enrichment recovery (above 79%). Furthermore, the proposed material was successfully utilized in the N-glycoproteomics analysis of a complex biological sample. 1305 unique glycopeptides with 883 N-glycosylation sites from 476 different N-linked glycoproteins were identified from the proteins extracted from mouse liver. These results show that the proposed Au-cysteine modified macroporous adsorption resin is a promising affinity material for glycoproteomics research of real complex biological samples.
Language英语
WOS IDWOS:000389905300039
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150426
Collection中国科学院大连化学物理研究所
Affiliation1.East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Sun, Xudong,Dong, Jing,Li, Jinan,et al. Au-cysteine modified macroporous adsorption resin: preparation and highly selective enrichment and identification of N-linked glycopeptides from the complex biological sample[J]. RSC ADVANCES,2016,6(114):113058-113065.
APA Sun, Xudong.,Dong, Jing.,Li, Jinan.,Ye, Mingliang.,Ou, Junjie.,...&Zhang, Weibing.(2016).Au-cysteine modified macroporous adsorption resin: preparation and highly selective enrichment and identification of N-linked glycopeptides from the complex biological sample.RSC ADVANCES,6(114),113058-113065.
MLA Sun, Xudong,et al."Au-cysteine modified macroporous adsorption resin: preparation and highly selective enrichment and identification of N-linked glycopeptides from the complex biological sample".RSC ADVANCES 6.114(2016):113058-113065.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Sun, Xudong]'s Articles
[Dong, Jing]'s Articles
[Li, Jinan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Sun, Xudong]'s Articles
[Dong, Jing]'s Articles
[Li, Jinan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sun, Xudong]'s Articles
[Dong, Jing]'s Articles
[Li, Jinan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.