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A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells
Kong, Jing1; Luo, Yong2; Jin, Dong1; An, Fan2; Zhang, Wenyuan1; Liu, Lilu1; Li, Jiao1; Fang, Shimeng1; Li, Xiaojie1; Yang, Xuesong3; Lin, Bingcheng2,4; Liu, Tingjiao1
关键词Microfluidic Metastasis Circulating Tumor Cells Multi-organ Bionic Model
刊名ONCOTARGET
2016-11-29
DOI10.18632/oncotarget.9382
7期:48页:78421-78432
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Oncology ; Cell Biology
关键词[WOS]BREAST-CANCER METASTASIS ; ENDOTHELIAL BARRIER FUNCTION ; ANIMAL-MODELS ; IN-VIVO ; CHIP ; CULTURE ; FLOW ; PROGRESSION ; MIGRATION ; PATTERNS
英文摘要A biomimetic microsystem might compensate costly and time-consuming animal metastatic models. Herein we developed a biomimetic microfluidic model to study cancer metastasis. Primary cells isolated from different organs were cultured on the microlfuidic model to represent individual organs. Breast and salivary gland cancer cells were driven to flow over primary cell culture chambers, mimicking dynamic adhesion of circulating tumor cells (CTCs) to endothelium in vivo. These flowing artificial CTCs showed different metastatic potentials to lung on the microfluidic model. The traditional nude mouse model of lung metastasis was performed to investigate the physiological similarity of the microfluidic model to animal models. It was found that the metastatic potential of different cancer cells assessed by the microfluidic model was in agreement with that assessed by the nude mouse model. Furthermore, it was demonstrated that the metastatic inhibitor AMD3100 inhibited lung metastasis effectively in both the microfluidic model and the nude mouse model. Then the microfluidic model was used to mimick liver and bone metastasis of CTCs and confirm the potential for research of multiple-organ metastasis. Thus, the metastasis of CTCs to different organs was reconstituted on the microfluidic model. It may expand the capabilities of traditional cell culture models, providing a low-cost, time-saving, and rapid alternative to animal models.
语种英语
WOS记录号WOS:000389636000020
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/150428
专题中国科学院大连化学物理研究所
作者单位1.Dalian Med Univ, Coll Stomatol, Dalian, Peoples R China
2.Dalian Univ Technol, Fac Chem Environm & Biol Sci & Technol, Dalian, Peoples R China
3.Dalian Med Univ, Liaoning Prov Core Lab Glycobiol & Glycoengn, Dept Biochem & Mol Biol, Dalian, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China
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Kong, Jing,Luo, Yong,Jin, Dong,et al. A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells[J]. ONCOTARGET,2016,7(48):78421-78432.
APA Kong, Jing.,Luo, Yong.,Jin, Dong.,An, Fan.,Zhang, Wenyuan.,...&Liu, Tingjiao.(2016).A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells.ONCOTARGET,7(48),78421-78432.
MLA Kong, Jing,et al."A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells".ONCOTARGET 7.48(2016):78421-78432.
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