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题名: A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells
作者: Kong, Jing1;  Luo, Yong2;  Jin, Dong1;  An, Fan2;  Zhang, Wenyuan1;  Liu, Lilu1;  Li, Jiao1;  Fang, Shimeng1;  Li, Xiaojie1;  Yang, Xuesong3;  Lin, Bingcheng2, 4;  Liu, Tingjiao1
关键词: microfluidic ;  metastasis ;  circulating tumor cells ;  multi-organ ;  bionic model
刊名: ONCOTARGET
发表日期: 2016-11-29
DOI: 10.18632/oncotarget.9382
卷: 7, 期:48, 页:78421-78432
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Oncology ;  Cell Biology
英文摘要: A biomimetic microsystem might compensate costly and time-consuming animal metastatic models. Herein we developed a biomimetic microfluidic model to study cancer metastasis. Primary cells isolated from different organs were cultured on the microlfuidic model to represent individual organs. Breast and salivary gland cancer cells were driven to flow over primary cell culture chambers, mimicking dynamic adhesion of circulating tumor cells (CTCs) to endothelium in vivo. These flowing artificial CTCs showed different metastatic potentials to lung on the microfluidic model. The traditional nude mouse model of lung metastasis was performed to investigate the physiological similarity of the microfluidic model to animal models. It was found that the metastatic potential of different cancer cells assessed by the microfluidic model was in agreement with that assessed by the nude mouse model. Furthermore, it was demonstrated that the metastatic inhibitor AMD3100 inhibited lung metastasis effectively in both the microfluidic model and the nude mouse model. Then the microfluidic model was used to mimick liver and bone metastasis of CTCs and confirm the potential for research of multiple-organ metastasis. Thus, the metastasis of CTCs to different organs was reconstituted on the microfluidic model. It may expand the capabilities of traditional cell culture models, providing a low-cost, time-saving, and rapid alternative to animal models.
关键词[WOS]: BREAST-CANCER METASTASIS ;  ENDOTHELIAL BARRIER FUNCTION ;  ANIMAL-MODELS ;  IN-VIVO ;  CHIP ;  CULTURE ;  FLOW ;  PROGRESSION ;  MIGRATION ;  PATTERNS
语种: 英语
WOS记录号: WOS:000389636000020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150428
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, Coll Stomatol, Dalian, Peoples R China
2.Dalian Univ Technol, Fac Chem Environm & Biol Sci & Technol, Dalian, Peoples R China
3.Dalian Med Univ, Liaoning Prov Core Lab Glycobiol & Glycoengn, Dept Biochem & Mol Biol, Dalian, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China

Recommended Citation:
Kong, Jing,Luo, Yong,Jin, Dong,et al. A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells[J]. ONCOTARGET,2016,7(48):78421-78432.
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