DICP OpenIR
A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells
Kong, Jing1; Luo, Yong2; Jin, Dong1; An, Fan2; Zhang, Wenyuan1; Liu, Lilu1; Li, Jiao1; Fang, Shimeng1; Li, Xiaojie1; Yang, Xuesong3; Lin, Bingcheng2,4; Liu, Tingjiao1
KeywordMicrofluidic Metastasis Circulating Tumor Cells Multi-organ Bionic Model
Source PublicationONCOTARGET
2016-11-29
DOI10.18632/oncotarget.9382
Volume7Issue:48Pages:78421-78432
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectOncology ; Cell Biology
WOS KeywordBREAST-CANCER METASTASIS ; ENDOTHELIAL BARRIER FUNCTION ; ANIMAL-MODELS ; IN-VIVO ; CHIP ; CULTURE ; FLOW ; PROGRESSION ; MIGRATION ; PATTERNS
AbstractA biomimetic microsystem might compensate costly and time-consuming animal metastatic models. Herein we developed a biomimetic microfluidic model to study cancer metastasis. Primary cells isolated from different organs were cultured on the microlfuidic model to represent individual organs. Breast and salivary gland cancer cells were driven to flow over primary cell culture chambers, mimicking dynamic adhesion of circulating tumor cells (CTCs) to endothelium in vivo. These flowing artificial CTCs showed different metastatic potentials to lung on the microfluidic model. The traditional nude mouse model of lung metastasis was performed to investigate the physiological similarity of the microfluidic model to animal models. It was found that the metastatic potential of different cancer cells assessed by the microfluidic model was in agreement with that assessed by the nude mouse model. Furthermore, it was demonstrated that the metastatic inhibitor AMD3100 inhibited lung metastasis effectively in both the microfluidic model and the nude mouse model. Then the microfluidic model was used to mimick liver and bone metastasis of CTCs and confirm the potential for research of multiple-organ metastasis. Thus, the metastasis of CTCs to different organs was reconstituted on the microfluidic model. It may expand the capabilities of traditional cell culture models, providing a low-cost, time-saving, and rapid alternative to animal models.
Language英语
WOS IDWOS:000389636000020
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150428
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Med Univ, Coll Stomatol, Dalian, Peoples R China
2.Dalian Univ Technol, Fac Chem Environm & Biol Sci & Technol, Dalian, Peoples R China
3.Dalian Med Univ, Liaoning Prov Core Lab Glycobiol & Glycoengn, Dept Biochem & Mol Biol, Dalian, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Kong, Jing,Luo, Yong,Jin, Dong,et al. A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells[J]. ONCOTARGET,2016,7(48):78421-78432.
APA Kong, Jing.,Luo, Yong.,Jin, Dong.,An, Fan.,Zhang, Wenyuan.,...&Liu, Tingjiao.(2016).A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells.ONCOTARGET,7(48),78421-78432.
MLA Kong, Jing,et al."A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells".ONCOTARGET 7.48(2016):78421-78432.
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