DICP OpenIR
Gold-Coated Nanoelectrospray Emitters Fabricated by Gravity-Assisted Etching Self-Termination and Electroless Deposition
Zhu, Xudong1,2; Liang, Yu1; Weng, Yejing1,2; Chen, Yuanbo1,2; Jiang, Hao1,2; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1
Source PublicationANALYTICAL CHEMISTRY
2016-12-06
DOI10.1021/acs.analchem.6b03422
Volume88Issue:23Pages:11347-11351
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS KeywordIONIZATION MASS-SPECTROMETRY ; CAPILLARY COLUMN ; ESI-MS ; ELECTROSPRAY ; PROTEOME ; SURFACE
AbstractTo improve the stability and sensitivity of nanoelectrospray for liquid chromatography-mass spectroscopy (LC-MS) analysis, we present a new method to fabricate gold-coated emitters. Via gravity-assisted etching self-termination, the emitter with a tapered outer surface and a straight inner surface is prepared with good reproducibility, without the need of fluid introduced to protect internal surface during etching. Followed by electroless deposition, the emitter is further coated with gold film homogeneously, by which the relative standard deviation (RSD) value of total ion current in 160 h is <5%, showing good stability. Compared to that obtained by a commercial emitter, the identified protein number from 2 mu g HeLa cell digests is increased over 10%, contributed by the stable electrospray and improved signal intensity of peptides. Furthermore, the integrated gold-coated emitter is prepared at the end of the ultranarrow-bore packed column (inner diameter of 25 mu m), and 218 proteins are identified from 2 ng HeLa cell digests. All of these results demonstrate the great promise of such emitters for use in ultrasensitive proteome analysis.
Language英语
WOS IDWOS:000389556900016
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/150434
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Xudong,Liang, Yu,Weng, Yejing,et al. Gold-Coated Nanoelectrospray Emitters Fabricated by Gravity-Assisted Etching Self-Termination and Electroless Deposition[J]. ANALYTICAL CHEMISTRY,2016,88(23):11347-11351.
APA Zhu, Xudong.,Liang, Yu.,Weng, Yejing.,Chen, Yuanbo.,Jiang, Hao.,...&Zhang, Yukui.(2016).Gold-Coated Nanoelectrospray Emitters Fabricated by Gravity-Assisted Etching Self-Termination and Electroless Deposition.ANALYTICAL CHEMISTRY,88(23),11347-11351.
MLA Zhu, Xudong,et al."Gold-Coated Nanoelectrospray Emitters Fabricated by Gravity-Assisted Etching Self-Termination and Electroless Deposition".ANALYTICAL CHEMISTRY 88.23(2016):11347-11351.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhu, Xudong]'s Articles
[Liang, Yu]'s Articles
[Weng, Yejing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhu, Xudong]'s Articles
[Liang, Yu]'s Articles
[Weng, Yejing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhu, Xudong]'s Articles
[Liang, Yu]'s Articles
[Weng, Yejing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.