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Gold-Coated Nanoelectrospray Emitters Fabricated by Gravity-Assisted Etching Self-Termination and Electroless Deposition
Zhu, Xudong1,2; Liang, Yu1; Weng, Yejing1,2; Chen, Yuanbo1,2; Jiang, Hao1,2; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1
刊名ANALYTICAL CHEMISTRY
2016-12-06
DOI10.1021/acs.analchem.6b03422
88期:23页:11347-11351
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
关键词[WOS]IONIZATION MASS-SPECTROMETRY ; CAPILLARY COLUMN ; ESI-MS ; ELECTROSPRAY ; PROTEOME ; SURFACE
英文摘要To improve the stability and sensitivity of nanoelectrospray for liquid chromatography-mass spectroscopy (LC-MS) analysis, we present a new method to fabricate gold-coated emitters. Via gravity-assisted etching self-termination, the emitter with a tapered outer surface and a straight inner surface is prepared with good reproducibility, without the need of fluid introduced to protect internal surface during etching. Followed by electroless deposition, the emitter is further coated with gold film homogeneously, by which the relative standard deviation (RSD) value of total ion current in 160 h is <5%, showing good stability. Compared to that obtained by a commercial emitter, the identified protein number from 2 mu g HeLa cell digests is increased over 10%, contributed by the stable electrospray and improved signal intensity of peptides. Furthermore, the integrated gold-coated emitter is prepared at the end of the ultranarrow-bore packed column (inner diameter of 25 mu m), and 218 proteins are identified from 2 ng HeLa cell digests. All of these results demonstrate the great promise of such emitters for use in ultrasensitive proteome analysis.
语种英语
WOS记录号WOS:000389556900016
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/150434
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
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GB/T 7714
Zhu, Xudong,Liang, Yu,Weng, Yejing,et al. Gold-Coated Nanoelectrospray Emitters Fabricated by Gravity-Assisted Etching Self-Termination and Electroless Deposition[J]. ANALYTICAL CHEMISTRY,2016,88(23):11347-11351.
APA Zhu, Xudong.,Liang, Yu.,Weng, Yejing.,Chen, Yuanbo.,Jiang, Hao.,...&Zhang, Yukui.(2016).Gold-Coated Nanoelectrospray Emitters Fabricated by Gravity-Assisted Etching Self-Termination and Electroless Deposition.ANALYTICAL CHEMISTRY,88(23),11347-11351.
MLA Zhu, Xudong,et al."Gold-Coated Nanoelectrospray Emitters Fabricated by Gravity-Assisted Etching Self-Termination and Electroless Deposition".ANALYTICAL CHEMISTRY 88.23(2016):11347-11351.
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