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题名: Gold-Coated Nanoelectrospray Emitters Fabricated by Gravity-Assisted Etching Self-Termination and Electroless Deposition
作者: Zhu, Xudong1, 2;  Liang, Yu1;  Weng, Yejing1, 2;  Chen, Yuanbo1, 2;  Jiang, Hao1, 2;  Zhang, Lihua1;  Liang, Zhen1;  Zhang, Yukui1
刊名: ANALYTICAL CHEMISTRY
发表日期: 2016-12-06
DOI: 10.1021/acs.analchem.6b03422
卷: 88, 期:23, 页:11347-11351
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
英文摘要: To improve the stability and sensitivity of nanoelectrospray for liquid chromatography-mass spectroscopy (LC-MS) analysis, we present a new method to fabricate gold-coated emitters. Via gravity-assisted etching self-termination, the emitter with a tapered outer surface and a straight inner surface is prepared with good reproducibility, without the need of fluid introduced to protect internal surface during etching. Followed by electroless deposition, the emitter is further coated with gold film homogeneously, by which the relative standard deviation (RSD) value of total ion current in 160 h is <5%, showing good stability. Compared to that obtained by a commercial emitter, the identified protein number from 2 mu g HeLa cell digests is increased over 10%, contributed by the stable electrospray and improved signal intensity of peptides. Furthermore, the integrated gold-coated emitter is prepared at the end of the ultranarrow-bore packed column (inner diameter of 25 mu m), and 218 proteins are identified from 2 ng HeLa cell digests. All of these results demonstrate the great promise of such emitters for use in ultrasensitive proteome analysis.
关键词[WOS]: IONIZATION MASS-SPECTROMETRY ;  CAPILLARY COLUMN ;  ESI-MS ;  ELECTROSPRAY ;  PROTEOME ;  SURFACE
语种: 英语
WOS记录号: WOS:000389556900016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/150434
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China

Recommended Citation:
Zhu, Xudong,Liang, Yu,Weng, Yejing,et al. Gold-Coated Nanoelectrospray Emitters Fabricated by Gravity-Assisted Etching Self-Termination and Electroless Deposition[J]. ANALYTICAL CHEMISTRY,2016,88(23):11347-11351.
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