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Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids
Wang, Yanan1,3; Zhang, Sufang1; Potter, Markus2; Sun, Wenyi1; Li, Li2; Yang, Xiaobing1; Jiao, Xiang1; Zhao, Zongbao K.1
关键词Rhodosporidium Toruloides Delta 12-fatty Acid Desaturase Linoleic Acid Microbial Lipids Genetic Engineering
刊名APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
2016-12-01
DOI10.1007/s12010-016-2182-9
180期:8页:1497-1507
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]SACCHAROMYCES-CEREVISIAE ; YARROWIA-LIPOLYTICA ; HETEROLOGOUS EXPRESSION ; TRANSFORMATION ; GENES ; IDENTIFICATION ; AGROBACTERIUM ; ACCUMULATION ; TOLERANCE ; CLONING
英文摘要Under nutrient-limited conditions, the red yeast Rhodosporidium toruloides can accumulate neutral lipids, of which the compositional fatty acids are mainly saturated and mono-unsaturated ones with 16 or 18 carbon atoms. To improve the linoleic acid content in the lipids, we enabled galactose-inducible expression of the gene encoding Delta 12-fatty acid desaturase (FADS) from Mortierella alpina or Fusarium verticillioides by integration of the corresponding expression cassettes into the genome of R. toruloides haploid and diploid strains. The relative linoleic acid content increased up to fivefold and the final linoleic acid titer reached 1.3 g/L under flask culture conditions. Our results suggested that R. toruloides may be further explored as cell factory for production of high-valued lipids and other fatty acid derivatives as bio-based chemicals and fuels.
语种英语
WOS记录号WOS:000389892200003
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被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/151823
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Evon Degussa China Co Ltd, Shanghai Branch, Shanghai 201108, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Wang, Yanan,Zhang, Sufang,Potter, Markus,et al. Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2016,180(8):1497-1507.
APA Wang, Yanan.,Zhang, Sufang.,Potter, Markus.,Sun, Wenyi.,Li, Li.,...&Zhao, Zongbao K..(2016).Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,180(8),1497-1507.
MLA Wang, Yanan,et al."Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 180.8(2016):1497-1507.
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