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题名: Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids
作者: Wang, Yanan1, 3;  Zhang, Sufang1;  Potter, Markus2;  Sun, Wenyi1;  Li, Li2;  Yang, Xiaobing1;  Jiao, Xiang1;  Zhao, Zongbao K.1
关键词: Rhodosporidium toruloides ;  Delta 12-fatty acid desaturase ;  Linoleic acid ;  Microbial lipids ;  Genetic engineering
刊名: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
发表日期: 2016-12-01
DOI: 10.1007/s12010-016-2182-9
卷: 180, 期:8, 页:1497-1507
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
英文摘要: Under nutrient-limited conditions, the red yeast Rhodosporidium toruloides can accumulate neutral lipids, of which the compositional fatty acids are mainly saturated and mono-unsaturated ones with 16 or 18 carbon atoms. To improve the linoleic acid content in the lipids, we enabled galactose-inducible expression of the gene encoding Delta 12-fatty acid desaturase (FADS) from Mortierella alpina or Fusarium verticillioides by integration of the corresponding expression cassettes into the genome of R. toruloides haploid and diploid strains. The relative linoleic acid content increased up to fivefold and the final linoleic acid titer reached 1.3 g/L under flask culture conditions. Our results suggested that R. toruloides may be further explored as cell factory for production of high-valued lipids and other fatty acid derivatives as bio-based chemicals and fuels.
关键词[WOS]: SACCHAROMYCES-CEREVISIAE ;  YARROWIA-LIPOLYTICA ;  HETEROLOGOUS EXPRESSION ;  TRANSFORMATION ;  GENES ;  IDENTIFICATION ;  AGROBACTERIUM ;  ACCUMULATION ;  TOLERANCE ;  CLONING
语种: 英语
WOS记录号: WOS:000389892200003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/151823
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Evon Degussa China Co Ltd, Shanghai Branch, Shanghai 201108, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Wang, Yanan,Zhang, Sufang,Potter, Markus,et al. Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2016,180(8):1497-1507.
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