DICP OpenIR
Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids
Wang, Yanan1,3; Zhang, Sufang1; Potter, Markus2; Sun, Wenyi1; Li, Li2; Yang, Xiaobing1; Jiao, Xiang1; Zhao, Zongbao K.1
KeywordRhodosporidium Toruloides Delta 12-fatty Acid Desaturase Linoleic Acid Microbial Lipids Genetic Engineering
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
2016-12-01
DOI10.1007/s12010-016-2182-9
Volume180Issue:8Pages:1497-1507
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS KeywordSACCHAROMYCES-CEREVISIAE ; YARROWIA-LIPOLYTICA ; HETEROLOGOUS EXPRESSION ; TRANSFORMATION ; GENES ; IDENTIFICATION ; AGROBACTERIUM ; ACCUMULATION ; TOLERANCE ; CLONING
AbstractUnder nutrient-limited conditions, the red yeast Rhodosporidium toruloides can accumulate neutral lipids, of which the compositional fatty acids are mainly saturated and mono-unsaturated ones with 16 or 18 carbon atoms. To improve the linoleic acid content in the lipids, we enabled galactose-inducible expression of the gene encoding Delta 12-fatty acid desaturase (FADS) from Mortierella alpina or Fusarium verticillioides by integration of the corresponding expression cassettes into the genome of R. toruloides haploid and diploid strains. The relative linoleic acid content increased up to fivefold and the final linoleic acid titer reached 1.3 g/L under flask culture conditions. Our results suggested that R. toruloides may be further explored as cell factory for production of high-valued lipids and other fatty acid derivatives as bio-based chemicals and fuels.
Language英语
WOS IDWOS:000389892200003
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/151823
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Evon Degussa China Co Ltd, Shanghai Branch, Shanghai 201108, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yanan,Zhang, Sufang,Potter, Markus,et al. Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2016,180(8):1497-1507.
APA Wang, Yanan.,Zhang, Sufang.,Potter, Markus.,Sun, Wenyi.,Li, Li.,...&Zhao, Zongbao K..(2016).Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,180(8),1497-1507.
MLA Wang, Yanan,et al."Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 180.8(2016):1497-1507.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Yanan]'s Articles
[Zhang, Sufang]'s Articles
[Potter, Markus]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Yanan]'s Articles
[Zhang, Sufang]'s Articles
[Potter, Markus]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Yanan]'s Articles
[Zhang, Sufang]'s Articles
[Potter, Markus]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.