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题名: Fluorescent trimethyl-substituted naphthyridine as a label-free signal reporter for one-step and highly sensitive fluorescent detection of DNA in serum samples
作者: Wang, Jiamian1, 2, 3;  Wang, Xiuyun1;  Wu, Shuo1, 2, 3;  Che, Ruping4;  Luo, Pinchen1;  Meng, Changgong1, 2, 3
关键词: ATMND ;  Label-free ;  Exo III assisted cascade target recycling amplification ;  C-C mismatch ;  Fluorescent DNA detection
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2017-01-15
DOI: 10.1016/j.bios.2016.09.064
卷: 87, 页:984-990
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry, Analytical ;  Electrochemistry ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry ;  Electrochemistry ;  Science & Technology - Other Topics
英文摘要: A facile label-free sensing method is developed for the one-step and highly sensitive fluorescent detection of DNA, which couples the specific C-C mismatch bonding and fluorescent quenching property of a trimethyl-substituted naphthyridine dye (ATMND) with the exonuclease III (Exo III) assisted cascade target recycling amplification strategy. In the absence of target DNA, the DNA hairpin probe with a C-C mismatch in the stem and more than 4 bases overhung at the 3' terminus could entrap and quench the fluorescence of ATMND and resist the digestion of Exo III, thus showing a low fluorescence background. In the presence of the target, however, the hybridization event between the two protruding segments and the target triggers the digestion reaction of Exo III, recycles the initial target, and simultaneously releases both the secondary target analogue and the ATMND caged in the stem. The released initial and secondary targets take part in another cycle of digestion, thus leading to the release of a huge amount of free ATMND for signal transducing. Based on the fluorescence recovery, the as-proposed label-free fluorescent sensing strategy shows very good analytical performances towards DNA detection, such as a wide linear range from 10 pM to 1 mu M, a low limit of detection of 6 pM, good selectivity, and a facile one-step operation at room temperature. Practical sample analysis in serum samples indicates the method has good precision and accuracy, which may thus have application potentials for point-of-care screening of DNA in complex clinical and environmental samples.
关键词[WOS]: ROLLING-CIRCLE AMPLIFICATION ;  TEMPLATED SILVER NANOCLUSTERS ;  SYBR GREEN I ;  EXONUCLEASE-III ;  NUCLEIC-ACID ;  RECYCLING AMPLIFICATION ;  ENERGY-TRANSFER ;  PYRENE-EXCIMER ;  CHAIN-REACTION ;  ASSAY
语种: 英语
WOS记录号: WOS:000390499600134
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/151831
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, Sch Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Zhang Dayu Sch Chem, Dalian, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
4.Dalian 7 Peoples Hosp, Dalian, Peoples R China

Recommended Citation:
Wang, Jiamian,Wang, Xiuyun,Wu, Shuo,et al. Fluorescent trimethyl-substituted naphthyridine as a label-free signal reporter for one-step and highly sensitive fluorescent detection of DNA in serum samples[J]. BIOSENSORS & BIOELECTRONICS,2017,87:984-990.
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