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Fluorescent trimethyl-substituted naphthyridine as a label-free signal reporter for one-step and highly sensitive fluorescent detection of DNA in serum samples
Wang, Jiamian1,2,3; Wang, Xiuyun1; Wu, Shuo1,2,3; Che, Ruping4; Luo, Pinchen1; Meng, Changgong1,2,3
关键词Atmnd Label-free Exo Iii Assisted Cascade Target Recycling Amplification C-c Mismatch Fluorescent Dna Detection
刊名BIOSENSORS & BIOELECTRONICS
2017-01-15
DOI10.1016/j.bios.2016.09.064
87页:984-990
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
研究领域[WOS]Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
关键词[WOS]ROLLING-CIRCLE AMPLIFICATION ; TEMPLATED SILVER NANOCLUSTERS ; SYBR GREEN I ; EXONUCLEASE-III ; NUCLEIC-ACID ; RECYCLING AMPLIFICATION ; ENERGY-TRANSFER ; PYRENE-EXCIMER ; CHAIN-REACTION ; ASSAY
英文摘要A facile label-free sensing method is developed for the one-step and highly sensitive fluorescent detection of DNA, which couples the specific C-C mismatch bonding and fluorescent quenching property of a trimethyl-substituted naphthyridine dye (ATMND) with the exonuclease III (Exo III) assisted cascade target recycling amplification strategy. In the absence of target DNA, the DNA hairpin probe with a C-C mismatch in the stem and more than 4 bases overhung at the 3' terminus could entrap and quench the fluorescence of ATMND and resist the digestion of Exo III, thus showing a low fluorescence background. In the presence of the target, however, the hybridization event between the two protruding segments and the target triggers the digestion reaction of Exo III, recycles the initial target, and simultaneously releases both the secondary target analogue and the ATMND caged in the stem. The released initial and secondary targets take part in another cycle of digestion, thus leading to the release of a huge amount of free ATMND for signal transducing. Based on the fluorescence recovery, the as-proposed label-free fluorescent sensing strategy shows very good analytical performances towards DNA detection, such as a wide linear range from 10 pM to 1 mu M, a low limit of detection of 6 pM, good selectivity, and a facile one-step operation at room temperature. Practical sample analysis in serum samples indicates the method has good precision and accuracy, which may thus have application potentials for point-of-care screening of DNA in complex clinical and environmental samples.
语种英语
WOS记录号WOS:000390499600134
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/151831
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, Sch Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Zhang Dayu Sch Chem, Dalian, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
4.Dalian 7 Peoples Hosp, Dalian, Peoples R China
推荐引用方式
GB/T 7714
Wang, Jiamian,Wang, Xiuyun,Wu, Shuo,et al. Fluorescent trimethyl-substituted naphthyridine as a label-free signal reporter for one-step and highly sensitive fluorescent detection of DNA in serum samples[J]. BIOSENSORS & BIOELECTRONICS,2017,87:984-990.
APA Wang, Jiamian,Wang, Xiuyun,Wu, Shuo,Che, Ruping,Luo, Pinchen,&Meng, Changgong.(2017).Fluorescent trimethyl-substituted naphthyridine as a label-free signal reporter for one-step and highly sensitive fluorescent detection of DNA in serum samples.BIOSENSORS & BIOELECTRONICS,87,984-990.
MLA Wang, Jiamian,et al."Fluorescent trimethyl-substituted naphthyridine as a label-free signal reporter for one-step and highly sensitive fluorescent detection of DNA in serum samples".BIOSENSORS & BIOELECTRONICS 87(2017):984-990.
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