DICP OpenIR
Fluorescent trimethyl-substituted naphthyridine as a label-free signal reporter for one-step and highly sensitive fluorescent detection of DNA in serum samples
Wang, Jiamian1,2,3; Wang, Xiuyun1; Wu, Shuo1,2,3; Che, Ruping4; Luo, Pinchen1; Meng, Changgong1,2,3
KeywordAtmnd Label-free Exo Iii Assisted Cascade Target Recycling Amplification C-c Mismatch Fluorescent Dna Detection
Source PublicationBIOSENSORS & BIOELECTRONICS
2017-01-15
DOI10.1016/j.bios.2016.09.064
Volume87Pages:984-990
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS KeywordROLLING-CIRCLE AMPLIFICATION ; TEMPLATED SILVER NANOCLUSTERS ; SYBR GREEN I ; EXONUCLEASE-III ; NUCLEIC-ACID ; RECYCLING AMPLIFICATION ; ENERGY-TRANSFER ; PYRENE-EXCIMER ; CHAIN-REACTION ; ASSAY
AbstractA facile label-free sensing method is developed for the one-step and highly sensitive fluorescent detection of DNA, which couples the specific C-C mismatch bonding and fluorescent quenching property of a trimethyl-substituted naphthyridine dye (ATMND) with the exonuclease III (Exo III) assisted cascade target recycling amplification strategy. In the absence of target DNA, the DNA hairpin probe with a C-C mismatch in the stem and more than 4 bases overhung at the 3' terminus could entrap and quench the fluorescence of ATMND and resist the digestion of Exo III, thus showing a low fluorescence background. In the presence of the target, however, the hybridization event between the two protruding segments and the target triggers the digestion reaction of Exo III, recycles the initial target, and simultaneously releases both the secondary target analogue and the ATMND caged in the stem. The released initial and secondary targets take part in another cycle of digestion, thus leading to the release of a huge amount of free ATMND for signal transducing. Based on the fluorescence recovery, the as-proposed label-free fluorescent sensing strategy shows very good analytical performances towards DNA detection, such as a wide linear range from 10 pM to 1 mu M, a low limit of detection of 6 pM, good selectivity, and a facile one-step operation at room temperature. Practical sample analysis in serum samples indicates the method has good precision and accuracy, which may thus have application potentials for point-of-care screening of DNA in complex clinical and environmental samples.
Language英语
WOS IDWOS:000390499600134
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/151831
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Univ Technol, Sch Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Zhang Dayu Sch Chem, Dalian, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
4.Dalian 7 Peoples Hosp, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Wang, Jiamian,Wang, Xiuyun,Wu, Shuo,et al. Fluorescent trimethyl-substituted naphthyridine as a label-free signal reporter for one-step and highly sensitive fluorescent detection of DNA in serum samples[J]. BIOSENSORS & BIOELECTRONICS,2017,87:984-990.
APA Wang, Jiamian,Wang, Xiuyun,Wu, Shuo,Che, Ruping,Luo, Pinchen,&Meng, Changgong.(2017).Fluorescent trimethyl-substituted naphthyridine as a label-free signal reporter for one-step and highly sensitive fluorescent detection of DNA in serum samples.BIOSENSORS & BIOELECTRONICS,87,984-990.
MLA Wang, Jiamian,et al."Fluorescent trimethyl-substituted naphthyridine as a label-free signal reporter for one-step and highly sensitive fluorescent detection of DNA in serum samples".BIOSENSORS & BIOELECTRONICS 87(2017):984-990.
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