DICP OpenIR
Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides
Peng, Jiaxi1,3; Zhang, Hongyan1,3; Li, Xin1,3; Liu, Shengju1,3; Zhao, Xingyun1,3; Wu, Jing4; Kang, Xiaohui1; Qin, Hongqiang1; Pan, Zaifa2; Wu, Ren'an1
关键词Metal-affinity Probe Immobilized Metal Ion Affinity Metal Oxide Affinity Metal-organic Framework Phosphorylated Peptide
刊名ACS APPLIED MATERIALS & INTERFACES
2016-12-28
DOI10.1021/acsami.6b12630
8期:51页:35012-35020
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Technology
类目[WOS]Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
研究领域[WOS]Science & Technology - Other Topics ; Materials Science
关键词[WOS]MASS-SPECTROMETRIC ANALYSIS ; SELECTIVE ENRICHMENT ; PHOSPHOPROTEOME ANALYSIS ; PHOSPHORYLATED PEPTIDES ; FACILE SYNTHESIS ; MAGNETIC NANOPARTICLES ; SIGNALING NETWORKS ; COMPLEX-MIXTURES ; MICROSPHERES ; CHROMATOGRAPHY
英文摘要The highly specific affinity between probes and phosphopeptides is the fundamental interaction for selective identification of phosphoproteomes that uncover the mechanisms of signal transduction, cell cycle, enzymatic regulation, and gene expression in biological systems. In this study, a metal-affinity probe possessing both interactions of metal oxide affinity chromatography (MOAC) and immobilized metal ion affinity chromatography (IMAC) was facilely prepared by immobilizing zirconium(W) on a zirconium organic framework of UiO-66-NH2, which holds dual metal centers of not only the inherent Zr-O cluster but also the immobilized Zr(IV) center. This dual-metal centered zirconium-organic framework (DZMOF) demonstrates as a highly specific metal-affinity probe toward the extraction of phosphopeptides due to the metal-affinity interactions of MOAC and IMAC toward either mono-phosphorylated or multi-phosphorylated peptides. The binding energies of zirconium 3d(5/2) and 3d(3/2) in this DZMOF are 183.07 and 185.47 eV, respectively, which are higher than those of the intact UiO-66-NH2 (182.84 and 185.17 eV, respectively), confirming the higher metal-affinity interaction between the DZMOF and phosphopeptides. This high metal-affinity probe presents an unprecedented strong performance in anti nonspecific interference during the capturing of phosphopeptides of beta-casein with the molar ratio of beta-casein vs bovine serum albumin up to ca. 1:5000. The enrichment of phosphopeptides from a human saliva sample by DZMOF further confirms the great potential of DZMOF in the extraction of low-abundance phosphopeptides for real complex biological samples.
语种英语
WOS记录号WOS:000391081700009
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/151845
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Zhejiang Univ Technol, Coll Chem Engn & Mat Sci, Hangzhou 310014, Zhejiang, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Wenzhou Inst Biomat & Engn, Wenzhou 325000, Peoples R China
推荐引用方式
GB/T 7714
Peng, Jiaxi,Zhang, Hongyan,Li, Xin,et al. Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(51):35012-35020.
APA Peng, Jiaxi.,Zhang, Hongyan.,Li, Xin.,Liu, Shengju.,Zhao, Xingyun.,...&Wu, Ren'an.(2016).Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides.ACS APPLIED MATERIALS & INTERFACES,8(51),35012-35020.
MLA Peng, Jiaxi,et al."Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides".ACS APPLIED MATERIALS & INTERFACES 8.51(2016):35012-35020.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Peng, Jiaxi]的文章
[Zhang, Hongyan]的文章
[Li, Xin]的文章
百度学术
百度学术中相似的文章
[Peng, Jiaxi]的文章
[Zhang, Hongyan]的文章
[Li, Xin]的文章
必应学术
必应学术中相似的文章
[Peng, Jiaxi]的文章
[Zhang, Hongyan]的文章
[Li, Xin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。