中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides
作者: Peng, Jiaxi1, 3;  Zhang, Hongyan1, 3;  Li, Xin1, 3;  Liu, Shengju1, 3;  Zhao, Xingyun1, 3;  Wu, Jing4;  Kang, Xiaohui1;  Qin, Hongqiang1;  Pan, Zaifa2;  Wu, Ren'an1
关键词: metal-affinity probe ;  immobilized metal ion affinity ;  metal oxide affinity ;  metal-organic framework ;  phosphorylated peptide
刊名: ACS APPLIED MATERIALS & INTERFACES
发表日期: 2016-12-28
DOI: 10.1021/acsami.6b12630
卷: 8, 期:51, 页:35012-35020
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Nanoscience & Nanotechnology ;  Materials Science, Multidisciplinary
研究领域[WOS]: Science & Technology - Other Topics ;  Materials Science
英文摘要: The highly specific affinity between probes and phosphopeptides is the fundamental interaction for selective identification of phosphoproteomes that uncover the mechanisms of signal transduction, cell cycle, enzymatic regulation, and gene expression in biological systems. In this study, a metal-affinity probe possessing both interactions of metal oxide affinity chromatography (MOAC) and immobilized metal ion affinity chromatography (IMAC) was facilely prepared by immobilizing zirconium(W) on a zirconium organic framework of UiO-66-NH2, which holds dual metal centers of not only the inherent Zr-O cluster but also the immobilized Zr(IV) center. This dual-metal centered zirconium-organic framework (DZMOF) demonstrates as a highly specific metal-affinity probe toward the extraction of phosphopeptides due to the metal-affinity interactions of MOAC and IMAC toward either mono-phosphorylated or multi-phosphorylated peptides. The binding energies of zirconium 3d(5/2) and 3d(3/2) in this DZMOF are 183.07 and 185.47 eV, respectively, which are higher than those of the intact UiO-66-NH2 (182.84 and 185.17 eV, respectively), confirming the higher metal-affinity interaction between the DZMOF and phosphopeptides. This high metal-affinity probe presents an unprecedented strong performance in anti nonspecific interference during the capturing of phosphopeptides of beta-casein with the molar ratio of beta-casein vs bovine serum albumin up to ca. 1:5000. The enrichment of phosphopeptides from a human saliva sample by DZMOF further confirms the great potential of DZMOF in the extraction of low-abundance phosphopeptides for real complex biological samples.
关键词[WOS]: MASS-SPECTROMETRIC ANALYSIS ;  SELECTIVE ENRICHMENT ;  PHOSPHOPROTEOME ANALYSIS ;  PHOSPHORYLATED PEPTIDES ;  FACILE SYNTHESIS ;  MAGNETIC NANOPARTICLES ;  SIGNALING NETWORKS ;  COMPLEX-MIXTURES ;  MICROSPHERES ;  CHROMATOGRAPHY
语种: 英语
WOS记录号: WOS:000391081700009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/151845
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Zhejiang Univ Technol, Coll Chem Engn & Mat Sci, Hangzhou 310014, Zhejiang, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Wenzhou Inst Biomat & Engn, Wenzhou 325000, Peoples R China

Recommended Citation:
Peng, Jiaxi,Zhang, Hongyan,Li, Xin,et al. Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(51):35012-35020.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Peng, Jiaxi]'s Articles
 [Zhang, Hongyan]'s Articles
 [Li, Xin]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Peng, Jiaxi]‘s Articles
 [Zhang, Hongyan]‘s Articles
 [Li, Xin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace