DICP OpenIR
Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides
Peng, Jiaxi1,3; Zhang, Hongyan1,3; Li, Xin1,3; Liu, Shengju1,3; Zhao, Xingyun1,3; Wu, Jing4; Kang, Xiaohui1; Qin, Hongqiang1; Pan, Zaifa2; Wu, Ren'an1
KeywordMetal-affinity Probe Immobilized Metal Ion Affinity Metal Oxide Affinity Metal-organic Framework Phosphorylated Peptide
Source PublicationACS APPLIED MATERIALS & INTERFACES
2016-12-28
DOI10.1021/acsami.6b12630
Volume8Issue:51Pages:35012-35020
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS KeywordMASS-SPECTROMETRIC ANALYSIS ; SELECTIVE ENRICHMENT ; PHOSPHOPROTEOME ANALYSIS ; PHOSPHORYLATED PEPTIDES ; FACILE SYNTHESIS ; MAGNETIC NANOPARTICLES ; SIGNALING NETWORKS ; COMPLEX-MIXTURES ; MICROSPHERES ; CHROMATOGRAPHY
AbstractThe highly specific affinity between probes and phosphopeptides is the fundamental interaction for selective identification of phosphoproteomes that uncover the mechanisms of signal transduction, cell cycle, enzymatic regulation, and gene expression in biological systems. In this study, a metal-affinity probe possessing both interactions of metal oxide affinity chromatography (MOAC) and immobilized metal ion affinity chromatography (IMAC) was facilely prepared by immobilizing zirconium(W) on a zirconium organic framework of UiO-66-NH2, which holds dual metal centers of not only the inherent Zr-O cluster but also the immobilized Zr(IV) center. This dual-metal centered zirconium-organic framework (DZMOF) demonstrates as a highly specific metal-affinity probe toward the extraction of phosphopeptides due to the metal-affinity interactions of MOAC and IMAC toward either mono-phosphorylated or multi-phosphorylated peptides. The binding energies of zirconium 3d(5/2) and 3d(3/2) in this DZMOF are 183.07 and 185.47 eV, respectively, which are higher than those of the intact UiO-66-NH2 (182.84 and 185.17 eV, respectively), confirming the higher metal-affinity interaction between the DZMOF and phosphopeptides. This high metal-affinity probe presents an unprecedented strong performance in anti nonspecific interference during the capturing of phosphopeptides of beta-casein with the molar ratio of beta-casein vs bovine serum albumin up to ca. 1:5000. The enrichment of phosphopeptides from a human saliva sample by DZMOF further confirms the great potential of DZMOF in the extraction of low-abundance phosphopeptides for real complex biological samples.
Language英语
WOS IDWOS:000391081700009
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/151845
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Zhejiang Univ Technol, Coll Chem Engn & Mat Sci, Hangzhou 310014, Zhejiang, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Wenzhou Inst Biomat & Engn, Wenzhou 325000, Peoples R China
Recommended Citation
GB/T 7714
Peng, Jiaxi,Zhang, Hongyan,Li, Xin,et al. Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(51):35012-35020.
APA Peng, Jiaxi.,Zhang, Hongyan.,Li, Xin.,Liu, Shengju.,Zhao, Xingyun.,...&Wu, Ren'an.(2016).Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides.ACS APPLIED MATERIALS & INTERFACES,8(51),35012-35020.
MLA Peng, Jiaxi,et al."Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides".ACS APPLIED MATERIALS & INTERFACES 8.51(2016):35012-35020.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Peng, Jiaxi]'s Articles
[Zhang, Hongyan]'s Articles
[Li, Xin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Peng, Jiaxi]'s Articles
[Zhang, Hongyan]'s Articles
[Li, Xin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Peng, Jiaxi]'s Articles
[Zhang, Hongyan]'s Articles
[Li, Xin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.