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题名: Facile Synthesis of Mesocrystalline SnO2 Nanorods on Reduced Graphene Oxide Sheets: An Appealing Multifunctional Affinity Probe for Sequential Enrichment of Endogenous Peptides and Phosphopeptides
作者: Ma, Wen1;  Zhang, Feng2;  Li, Liping3;  Chen, Shuai2;  Qi, Limin2;  Liu, Huwei1;  Bai, Yu1
关键词: phosphopeptides ;  endogenous peptides ;  multifunctional affinity probe ;  sequential enrichment ;  mass spectrometry
刊名: ACS APPLIED MATERIALS & INTERFACES
发表日期: 2016-12-28
DOI: 10.1021/acsami.6b14597
卷: 8, 期:51, 页:35099-35105
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Nanoscience & Nanotechnology ;  Materials Science, Multidisciplinary
研究领域[WOS]: Science & Technology - Other Topics ;  Materials Science
英文摘要: A novel multifunctional composite comprising mesocrystalline SnO, nanorods (NRs) vertically aligned on reduced graphene oxide (rGO) sheets was synthesized and developed for sequential capture of endogenous peptides and phosphopeptides. With the hydrophobicity of rGO and high affinity of SnO2 nanorods, sequential enrichment of endogenous peptides and phosphopeptides could be easily achieved through a modulation of elution buffer. With this multifunctional nanomaterial, 36 peptides were observed from diluted bovine serum albumin (BSA) tryptic digest and 4 phosphopeptides could be selectively captured from beta-casein digest. The detection limit of tryptic digest of beta-casein was low to 4 X 10(-10) M, and the selectivity was up to 1:500 (molar ratio of beta-casein and BSA digest). The effectiveness and robustness of rGO-SnO2 NRs in a complex biological system was also confirmed by using human serum as a real sample. Our work is promising for small peptide enrichment and identification especially in complicated biological sample preparation, which also opens a new perspective in the design of multifunctional affinity probes for proteome or peptidome.
关键词[WOS]: LITHIUM-ION BATTERIES ;  PHOSPHORYLATION SITES ;  CANCER BIOMARKERS ;  SELECTIVE CAPTURE ;  MASS-SPECTROMETRY ;  MS ANALYSIS ;  MICROSPHERES ;  PROTEIN ;  CARBON ;  MATRIX
语种: 英语
WOS记录号: WOS:000391081700017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/151848
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Coll Chem, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci,Minist Educ, Beijing 100871, Peoples R China
2.Peking Univ, Coll Chem, State Key Lab Struct Chem Stable & Unstable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
3.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China

Recommended Citation:
Ma, Wen,Zhang, Feng,Li, Liping,et al. Facile Synthesis of Mesocrystalline SnO2 Nanorods on Reduced Graphene Oxide Sheets: An Appealing Multifunctional Affinity Probe for Sequential Enrichment of Endogenous Peptides and Phosphopeptides[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(51):35099-35105.
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