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Facile Synthesis of Mesocrystalline SnO2 Nanorods on Reduced Graphene Oxide Sheets: An Appealing Multifunctional Affinity Probe for Sequential Enrichment of Endogenous Peptides and Phosphopeptides
Ma, Wen1; Zhang, Feng2; Li, Liping3; Chen, Shuai2; Qi, Limin2; Liu, Huwei1; Bai, Yu1
KeywordPhosphopeptides Endogenous Peptides Multifunctional Affinity Probe Sequential Enrichment Mass Spectrometry
Source PublicationACS APPLIED MATERIALS & INTERFACES
2016-12-28
DOI10.1021/acsami.6b14597
Volume8Issue:51Pages:35099-35105
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS KeywordLITHIUM-ION BATTERIES ; PHOSPHORYLATION SITES ; CANCER BIOMARKERS ; SELECTIVE CAPTURE ; MASS-SPECTROMETRY ; MS ANALYSIS ; MICROSPHERES ; PROTEIN ; CARBON ; MATRIX
AbstractA novel multifunctional composite comprising mesocrystalline SnO, nanorods (NRs) vertically aligned on reduced graphene oxide (rGO) sheets was synthesized and developed for sequential capture of endogenous peptides and phosphopeptides. With the hydrophobicity of rGO and high affinity of SnO2 nanorods, sequential enrichment of endogenous peptides and phosphopeptides could be easily achieved through a modulation of elution buffer. With this multifunctional nanomaterial, 36 peptides were observed from diluted bovine serum albumin (BSA) tryptic digest and 4 phosphopeptides could be selectively captured from beta-casein digest. The detection limit of tryptic digest of beta-casein was low to 4 X 10(-10) M, and the selectivity was up to 1:500 (molar ratio of beta-casein and BSA digest). The effectiveness and robustness of rGO-SnO2 NRs in a complex biological system was also confirmed by using human serum as a real sample. Our work is promising for small peptide enrichment and identification especially in complicated biological sample preparation, which also opens a new perspective in the design of multifunctional affinity probes for proteome or peptidome.
Language英语
WOS IDWOS:000391081700017
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/151848
Collection中国科学院大连化学物理研究所
Affiliation1.Peking Univ, Coll Chem, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci,Minist Educ, Beijing 100871, Peoples R China
2.Peking Univ, Coll Chem, State Key Lab Struct Chem Stable & Unstable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
3.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China
Recommended Citation
GB/T 7714
Ma, Wen,Zhang, Feng,Li, Liping,et al. Facile Synthesis of Mesocrystalline SnO2 Nanorods on Reduced Graphene Oxide Sheets: An Appealing Multifunctional Affinity Probe for Sequential Enrichment of Endogenous Peptides and Phosphopeptides[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(51):35099-35105.
APA Ma, Wen.,Zhang, Feng.,Li, Liping.,Chen, Shuai.,Qi, Limin.,...&Bai, Yu.(2016).Facile Synthesis of Mesocrystalline SnO2 Nanorods on Reduced Graphene Oxide Sheets: An Appealing Multifunctional Affinity Probe for Sequential Enrichment of Endogenous Peptides and Phosphopeptides.ACS APPLIED MATERIALS & INTERFACES,8(51),35099-35105.
MLA Ma, Wen,et al."Facile Synthesis of Mesocrystalline SnO2 Nanorods on Reduced Graphene Oxide Sheets: An Appealing Multifunctional Affinity Probe for Sequential Enrichment of Endogenous Peptides and Phosphopeptides".ACS APPLIED MATERIALS & INTERFACES 8.51(2016):35099-35105.
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